Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit | m/rAng-2 QKit
掲載日情報：2016/08/18 現在Webページ番号：111266
R&D Systems製のMouse/Rat Angiopoietin-2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月08日 09時55分現在]
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[在庫・価格 ：2024年05月08日 09時55分現在]
Mouse/Rat Angiopoietin-2 Quantikine ELISA Kit
文献数： 10
- 商品コード：MANG20
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：39.10 - 2500 pg/mL，感度：4.98 pg/mL，測定試料：Cell Culture Supernates (50 uL), Tissue Lysates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL)，種交差性：Mouse/Rat，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：Angiopoietin-2 別名：AGPT2 Genbank No： 285 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2019年12月15日号 p.6
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| 製品記事 |
Quantikine Angiopoietin-2 ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Lysates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 4.98 pg/mL
- Assay Range
- 39.1 - 2,500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Lysates)
- Specificity
- Natural and recombinant mouse and rat Angiopoietin-2.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse/Rat Angiopoietin-2 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse and rat Angiopoietin-2 in cell culture supernates, tissue lysates, serum, and plasma. It contains CHO cell-expressed recombinant mouse Angiopoietin-2 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural Angiopoietin-2 showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural Angiopoietin-2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Tissue Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 119 | 290 | 831 | 116 | 283 | 841 |
| Standard Deviation | 3.27 | 9.05 | 22.2 | 9.84 | 23.2 | 63.1 |
| CV% | 2.7 | 3.1 | 2.7 | 8.5 | 8.2 | 7.5 |
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Recovery
The recovery of Angiopoietin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 114 | 108-120 |
| Serum (n=2) | 113 | 109-117 |
| EDTA Plasma (n=2) | 105 | 101-110 |
| Heparin Plasma (n=2) | 107 | 103-111 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Angiopoietin-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Angiopoietin-2
- Aliases
- ANGPT2
- Entrez Gene IDs
- 285 (Human); 11601 (Mouse)
- Background
- Angiopoietins
- Angiopoietins, Ang-1, Ang-2, and Ang-3 (mouse)/Ang-4 (human), are natural agonists or antagonists of the Tie-2 receptor tyrosine kinase and are important modulators of angiogenesis. Two domains characterize the angiopoietin family of proteins: an N-terminal coiled-coil domain that mediates homo-oligomerization, and a C-terminal fibrinogen-like domain that binds Tie-2.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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