Mouse IL-6 Quantikine ELISA Kit | mIL-6 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111263
R&D Systems製の"Mouse IL-6"を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月05日 00時00分現在]
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IL-6, Mouse, ELISA Kit, Quantikine M, 2nd Generation (1Plate) |
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[在庫・価格 ：2024年05月05日 00時00分現在]
IL-6, Mouse, ELISA Kit, Quantikine M, 2nd Generation (1Plate)
文献数： 580
- 商品コード：M6000B-1
- メーカー：RSD
- 包装：1kit
- 価格：¥104,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：7.80 - 500 pg/mL，感度：1.8 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), Heparin Plasma (50 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-6 別名：B cell stimulatory factor-2 Genbank No： 3569 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine IL-6 ELISA Kit |
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| 関連記事 |
R&D Systems社製品#M6000Bの販売終了および後継品#M6000B-1の販売 R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 R&D Systems マウス／ラット用Quantikine ELISA Kit 2 plate製品の包装変更について |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 1.8 pg/mL
- Assay Range
- 7.8 - 500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates)
- Specificity
- Natural and recombinant mouse IL-6
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse IL-6 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-6 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-6 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-6. Results obtained using natural mouse IL-6 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-6.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 28 | 22 | 27 |
| Mean | 30 | 87 | 232 | 29 | 85 | 238 |
| Standard Deviation | 2 | 3.4 | 8.1 | 1.8 | 7.5 | 18.1 |
| CV% | 6.7 | 3.9 | 3.5 | 6.1 | 8.9 | 7.6 |
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Recovery
The recovery of mouse IL-6 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=5) | 110 | 86-120 |
| Serum (n=5) | 99 | 84-113 |
| Heparin Plasma (n=6) | 101 | 81-115 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of mouse IL-6 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-6
- Aliases
- BSF-2; IFN-beta 2; MGI-2A
- Entrez Gene IDs
- 3569 (Human); 16193 (Mouse); 24498 (Rat); 399500 (Porcine); 403985 (Canine); 100034196 (Equine); 493687 (Feline); 100008733 (Rabbit)
- Background
- IL-6
- Interleukin 6 (IL-6), also known as interferon-beta 2, 26 kDa protein and B cell stimulatory factor-2 (BSF-2), is a pleiotropic α-helical cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 activity is essential for the transition from acute inflammation to either acquired immunity or chronic inflammatory disease. It is secreted by multiple cell types as a 22 kDa - 28 kDa phosphorylated and variably glycosylated molecule. Mature human IL-6 is 183 amino acids (aa) in length and shares 41% aa sequence identity with mouse and rat IL-6. Alternate splicing generates several isoforms with internal deletions, some of which exhibit antagonistic properties.IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6 R) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R, triggering IL-6 R association with gp130 and gp130 dimerization. gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM. Soluble forms of IL-6 R are generated by both alternate splicing and proteolytic cleavage. In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R elicit responses from gp130-expressing cells that lack cell surface IL-6 R. Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R is predominantly restricted to hepatocytes, leukocytes, and lymphocytes. Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R but not from other cytokines that utilize gp130 as a coreceptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Sirt2 suppresses inflammatory responses in collagen-induced arthritis.
Hong H, Jiang C, Lin J, Sun B, Zheng Y
Biochem Biophys Res Commun 2013 441:897-903
Species: Mouse
Sample Type: Serum
Disruption of the chemokine-like receptor-1 (CMKLR1) gene is associated with reduced adiposity and glucose intolerance.
Butcher EC, Dranse HJ, Ernst MC, Haidl ID, Rourke JL, Sinal CJ, Z????iga LA, Zabel BA
Endocrinology 2012 153:672-82
Species: Mouse
Sample Type: Serum
Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
Aritake K, Hirai H, Nagata K, Nakamura M, Narumiya S, Otani S, Satoh T, Urade Y, Yamamoto Y, Yokozeki H
Am. J. Pathol. 2012 179:302-14
Species: Mouse
Sample Type: Tissue Homogenates
Toll-like receptor-mediated inhibition of Gas6 and ProS expression facilitates inflammatory cytokine production in mouse macrophages.
Chen Q, Deng T, Han D, Yan K, Zhang Y
Immunology 2012 135:40-50
Species: Mouse
Sample Type: Cell Culture Supernates
The axonal guidance receptor neogenin promotes acute inflammation.
Gatidou D, Granja T, Konig K, Meier J, Mirakaj V, Rosenberger P
PLoS ONE 2012 7:e32145
Species: Mouse
Sample Type: Peritoneal Lavage
Activated protein C inhibits pancreatic islet inflammation, stimulates T regulatory cells, and prevents diabetes in non-obese diabetic (NOD) mice.
Dervish S, Fulcher G, Harrison LC, Jackson CJ, Xue M
J. Biol. Chem. 2012 287:16356-64
Species: Mouse
Sample Type: Cell Culture Supernates
Absence of T and B lymphocytes modulates dystrophic features in dysferlin deficient animal model.
Belicchi M, Bottinelli R, D'Antona G, Del Fraro G, Farini A, Meregalli M, Navarro C, Parolini D, Razini P, Sitzia C, Torrente Y
Exp. Cell Res. 2012 318:1160-74
Species: Mouse
Sample Type: Tissue Homogenates
Cutting edge: Divergent cell-specific functions of MyD88 for inflammatory responses and organ injury in septic peritonitis.
Altmayr F, Gais P, Holzmann B, Janssen KP, Jusek G, Pfeffer K, Reim D, Rossmann-Bloeck T, Weighardt H
J. Immunol. 2012 188:5833-7
Species: Mouse
Sample Type: Plasma
Stress augments insulin resistance and prothrombotic state: role of visceral adipose-derived monocyte chemoattractant protein-1.
Cheng XW, Egashira K, Kikuchi R, Matsushita T, Murohara T, Nakamura H, Nakayama T, Nomura M, Takeshita K, Uchida Y, Yamamoto K
Diabetes 2012 61:1552-61
The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
Calderon J, Carrion J, Fresno M, Maganto-Garcia E, Punzon C, Terhorst C
PLoS Pathog. 2012 8:e1002799
Species: Mouse
Sample Type: Serum
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