Mouse IL-34 Quantikine ELISA Kit Kit | mIL-34 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111260
R&D Systems製のMouse IL-34 Kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月16日 00時00分現在]
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[在庫・価格 ：2025年05月16日 00時00分現在]
Mouse IL-34 Quantikine ELISA Kit Kit
文献数： 2
- 商品コード：M3400
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：39.1-2,500 pg/mL，感度：15.6 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Mouse，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-34 Genbank No： 146433 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | R＆D Systems社のIL-34関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 15.6 pg/mL
- Assay Range
- 39.1 - 2,500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse IL-34.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse IL-34 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-34 in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse IL-34 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-34. Results obtained using natural mouse IL-34 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-34.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 99.6 | 273 | 870 | 107 | 284 | 903 |
| Standard Deviation | 2.4 | 4 | 12.7 | 8.5 | 15.5 | 33.3 |
| CV% | 2.4 | 1.5 | 1.5 | 7.9 | 5.5 | 3.7 |
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Recovery
The recovery of IL-34 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 98 | 92-103 |
| Serum (n=4) | 100 | 95-107 |
| EDTA Plasma (n=4) | 100 | 85-109 |
| Heparin Plasma (n=4) | 103 | 99-110 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of IL-34 in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-34
- Aliases
- C16orf77
- Entrez Gene IDs
- 146433 (Human); 76527 (Mouse)
- Background
- IL-34
- Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level.IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen . The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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