Mouse IL-34 Quantikine ELISA Kit Kit | mIL-34 Qkit

掲載日情報:2016/08/18 現在Webページ番号:111260

R&D Systems製のMouse IL-34 Kitを測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2024年03月29日 17時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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Mouse IL-34 Quantikine ELISA Kit Kit
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 2
説明文
測定範囲:39.10 - 2500 pg/mL,感度:15.6 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL),種交差性:Mouse,測定波長:450 nm,検出方法:呈色,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,測定時間:4.5 hours,測定因子:IL-34
Genbank No: 146433
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 R&D Systems社のIL-34関連製品
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

[在庫・価格 :2024年03月29日 17時15分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Mouse IL-34 Quantikine ELISA Kit Kit

文献数: 2

説明文 測定範囲:39.10 - 2500 pg/mL,感度:15.6 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL),種交差性:Mouse,測定波長:450 nm,検出方法:呈色,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,測定時間:4.5 hours,測定因子:IL-34
Genbank No: 146433
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 R&D Systems社のIL-34関連製品
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity
15.6 pg/mL
Assay Range
39.1 - 2,500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant mouse IL-34.
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Mouse IL-34 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-34 in mouse cell culture supernates, tissue lysates, serum, and plasma. It contains NS0-expressed recombinant mouse IL-34 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-34. Results obtained using natural mouse IL-34 showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-34.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 99.6 273 870 107 284 903
Standard Deviation 2.4 4 12.7 8.5 15.5 33.3
CV% 2.4 1.5 1.5 7.9 5.5 3.7

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Recovery

The recovery of IL-34 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 98 92-103
Serum (n=4) 100 95-107
EDTA Plasma (n=4) 100 85-109
Heparin Plasma (n=4) 103 99-110

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Linearity

To assess the linearity of the assay, samples spiked with high concentrations of IL-34 in each matrix were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Additional Infomation

Molecule Information
IL-34
Aliases
C16orf77
Entrez Gene IDs
146433 (Human); 76527 (Mouse)
Background
IL-34
Interleukin 34 (IL-34; also known as uncharacterized protein C16orf77) is secreted as a homodimer consisting of 39 kDa monomers. It belongs to no known cytokine family. Human IL-34 is synthesized as a 242 amino acid (aa) precursor that contains a 20 aa signal sequence and a 222 aa mature chain. The mature chain contains one potential site of N-linked glycosylation. Human IL-34 is 71% identical to mouse IL-34 on the amino acid level.IL-34 is expressed in various tissues, including the heart, brain, liver, kidney, spleen, thymus, testes, ovary, small intestine, prostate, and colon, and is most abundant in the spleen . The receptor for IL-34 is colony-stimulating factor 1 receptor (CSF-1R). IL-34 stimulates monocyte proliferation. In functional studies, IL-34, like CSF-1, the other ligand for CSF-1R, stimulated phosphorylation of extracellular signal-regulated kinase-1 and -2 (ERK1/2) in human monocytes. In addition, IL-34 promoted the formation of the colony-forming unit-macrophage (CFU-M), a macrophage progenitor, in human bone marrow cultures.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

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