Mouse IL-17A/F Heterodimer Quantikine ELISA Kit | mIL-17A/F Qkit

掲載日情報:2016/08/18 現在Webページ番号:111253

R&D Systems製のMouse IL-17A/F Heterodimerを測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
IL-17A/F, Heterodimer, Mouse, ELISA Kit, Quantikine (96well)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 4
説明文
測定範囲:7.81 - 500 pg/mL,感度:2.8 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL),種交差性:Mouse,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,測定時間:4.5 hours,測定因子:IL-17A/F Heterodimer
別名:CTLA8
Genbank No: 3605
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Mouse IL-17A/F Heterodimer Quantikine® ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

[在庫・価格 :2024年05月04日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

IL-17A/F, Heterodimer, Mouse, ELISA Kit, Quantikine (96well)

文献数: 4

説明文 測定範囲:7.81 - 500 pg/mL,感度:2.8 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL),種交差性:Mouse,検出方法:呈色,測定波長:450 nm,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,測定時間:4.5 hours,測定因子:IL-17A/F Heterodimer
別名:CTLA8
Genbank No: 3605
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Mouse IL-17A/F Heterodimer Quantikine® ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)

Sensitivity
2.8 pg/mL
Assay Range
7.81 - 500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant mouse IL-17A/F heterodimer
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Mouse IL-17A/F Heterodimer immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-17A/F heterodimer in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-17A/F heterodimer and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant mouse IL-17A/F heterodimer. Results obtained using natural mouse IL-17A/F heterodimer showed dose response curves that were parallel to the standard curves obtained using the Quantikine mouse kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-17A/F heterodimer.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 22.8 119 180 25.1 112 188
Standard Deviation 1.4 5 11.1 2 9.8 11.6
CV% 6.1 4.2 6.2 8 8.8 6.2

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Recovery

The recovery of mouse IL-17A/F heterodimer spiked to three levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Serum (n=4) 91 84-98
Heparin Plasma (n=4) 91 86-98
EDTA Plasma (n=4) 99 90-104
Cell Culture Samples (n=4) 105 93-120

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Linearity

To assess the linearity of the assay, samples spiked with high concentrations of recombinant mouse IL-17A/F heterodimer in each matrix were diluted with Calibrator Diluent and assayed.

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Additional Infomation

Molecule Information
IL-17A/F Heterodimer
Aliases
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Entrez Gene IDs
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Background
IL-17 Ligands
The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

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