Mouse IL-17 Quantikine ELISA Kit | mIL-17 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111252
R&D Systems製のMouse IL-17を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月02日 00時00分現在]
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IL-17, Mouse, ELISA Kit, Quantikine M (1 Plate) |
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[在庫・価格 ：2024年05月02日 00時00分現在]
IL-17, Mouse, ELISA Kit, Quantikine M (1 Plate)
文献数： 133
- 商品コード：M1700-1
- メーカー：RSD
- 包装：1kit
- 価格：¥104,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：10.90 - 700 pg/mL，感度：5 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)，種交差性：Mouse，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：IL-17/IL-17A 別名：CTLA-8 Genbank No： 3605 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
R&D Systems Interleukin-17 (IL-17)測定キット |
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| 関連記事 |
R&D Systems社製品#M1700の販売終了および後継品#M1700-1の販売 R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 R&D Systems マウス／ラット用Quantikine ELISA Kit 2 plate製品の包装変更について |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 5 pg/mL
- Assay Range
- 10.9 - 700 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant mouse IL-17
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Mouse IL-17 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-17 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-17 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse IL-17 accurately. Results obtained using natural mouse IL-17 showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse IL-17.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 41.9 | 215 | 407 | 43.1 | 220 | 411 |
| Standard Deviation | 2.1 | 6.3 | 8.1 | 2.2 | 11.2 | 18.5 |
| CV% | 5 | 2.9 | 2 | 5.1 | 5.1 | 4.5 |
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Recovery
The recovery of mouse IL-17 spiked to three levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 102 | 91-108 |
| Heparin Plasma (n=3) | 101 | 91-123 |
| Cell Culture Supernates (n=6) | 101 | 92-118 |
| EDTA Plasma (n=3) | 107 | 93-120 |
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Linearity
To assess the linearity of the assay, five or more samples containing or spiked with various concentrations of mouse IL-17 in each matrix were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-17/IL-17A
- Aliases
- CTLA-8; IL17A
- Entrez Gene IDs
- 3605 (Human); 16171 (Mouse); 301289 (Rat); 481837 (Canine)
- Background
- IL-17 Ligands
- The IL-17 family is comprised of at least six proinflammatory cytokines that share a conserved cysteine-knot structure but diverge at the N-terminus. IL-17 family members are glycoproteins secreted as dimers that induce local cytokine production and recruit granulocytes to sites of inflammation. IL-17 is induced by IL-15 and IL-23, mainly in activated CD4+ T cells distinct from Th1 or Th2 cells. IL-17F is the most homologous to IL-17, but is induced only by IL-23 in activated monocytes. IL-17B binds the IL-17B receptor, but not the IL-17 receptor; it is most homologous with IL-17D, which is expressed by resting CD4+ T cells and CD19+ B cells. IL-17E is mainly produced by Th2 cells and recruits eosinophils to lung tissue. IL-17C has a very restricted expression pattern but has been detected in adult prostate and fetal kidney libraries.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Sirt2 suppresses inflammatory responses in collagen-induced arthritis.
Hong H, Jiang C, Lin J, Sun B, Zheng Y
Biochem Biophys Res Commun 2013 441:897-903
Species: Mouse
Sample Type: Serum
Dual functions of prostaglandin D2 in murine contact hypersensitivity via DP and CRTH2.
Aritake K, Hirai H, Nagata K, Nakamura M, Narumiya S, Otani S, Satoh T, Urade Y, Yamamoto Y, Yokozeki H
Am. J. Pathol. 2012 179:302-14
Species: Mouse
Sample Type: Tissue Homogenates
Salmonella enterica induces joint inflammation and expression of interleukin-17 in draining lymph nodes early after onset of enterocolitis in mice.
Cerquetti MC, Gartner AS, Giacomodonato MN, Noto Llana M, Sarnacki SH, Vazquez MV
Infect. Immun. 2012 80:2231-9
The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi.
Calderon J, Carrion J, Fresno M, Maganto-Garcia E, Punzon C, Terhorst C
PLoS Pathog. 2012 8:e1002799
Species: Mouse
Sample Type: Serum
The Role of IL-15 in Activating STAT5 and Fine-Tuning IL-17A Production in CD4 T Lymphocytes.
Ishihara S, Pandiyan P, Saravanamuthu S, Yang X, Zheng L
J. Immunol. 2012 189:4237-46
Elevating body temperature enhances hematopoiesis and neutrophil recovery after total body irradiation in an IL-1-, IL-17-, and G-CSF-dependent manner.
Blood 2012 120:2600-9
Species: Mouse
Sample Type: tissue lysates
Serotonin activates dendritic cell function in the context of gut inflammation.
Cote F, Ghia JE, Khan WI, Li N, Mallet J, McClemens J, Suehiro Y, Wang H
Am. J. Pathol. 2011 178:662-71
Species: Mouse
Sample Type: Cell Culture Supernates
Co-adjuvant effects of retinoic acid and IL-15 induce inflammatory immunity to dietary antigens.
Abadie V, DePaolo RW, Tang F
Nature 2011 471:220-4
Species: Mouse
Sample Type: Cell Culture Supernates
The TLR7 ligand 9-benzyl-2-butoxy-8-hydroxy adenine inhibits IL-17 response by eliciting IL-10 and IL-10-inducing cytokines.
Annunziato F, Guarna A, Maggi E, Maggi L, Nencini F, Parronchi P, Pratesi S, Romagnani S, Vultaggio A
J. Immunol. 2011 186:4707-15
Species: Mouse
Sample Type: Serum
IL-23-Mediated Epidermal Hyperplasia Is Dependent on IL-6.
Lindroos J, Norsgaard H, Svensson L
J. Invest. Dermatol. 2011 131:1110-8
Species: Mouse
Sample Type: Tissue Homogenates
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