Human FGF basic Quantikine HS ELISA Kit | hFGFb QKit HS Kit

R&D Systems製のHuman FGF basic Quantikine HS ELISA Kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。

The Quantikine HS Human FGF basic Immunoassay kit is a 5.5 hour solid phase ELISA designed to measure FGF basic levels in serum, plasma, and urine. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine HS kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine

The recovery of FGF basic spiked to three different levels throughout the range of the assay in various matrices was evaluated.

To assess the linearity of the assay, the following biological samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and assayed.

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

Plasma biomarkers as predictors of outcome in patients with advanced hepatocellular carcinoma.
Amadori D, Arvanitakis C, Badulescu F, Barrios C, Beaugrand M, Befeler A, Bennouna J, Blanc J-, Blum H, Bolondi L, Borbath I, Boyer M, Boyer T, Britten C, Bronowicki J-, Bruix J, Buggisch P, Burak K, Bustamante Schneider J, Byrne T, Caspary W, Cisnero Garza LE, Cognetti F, Cosme de Oliveira A, Craxi A, De Greve J, Degos F, Diago M, Dollinger M, Dominguez S, Falk S, Farinati F, Figer A, Forner A, Gamargo Garate C, Gane E, Garcia-Tsao G, Gerken G, Germanidis G, Goke B, Gold P, Goldenberg A, Grange J-, Gregor M, Greten T, Gridelli C, Haussinger D, Heuman D, Hilgard P, Hillon P, Jassem J, Jeffry Evans TR, Katsos I, Kennedy P, Koch A, Kotzev I, Kubyshkin V, Lathia CD, Leon J, Llovet JM, Lozano A, Ma M, Marrero J, Martinez-Castillo J, Martoni A, Mazzaferro V, Meinhardt G, Metrakos P, Meyer T, Miron L, Montero Alvarez JL, Muellhaupt B, O'Donnell A, Olweny C, Pallota MG, Pascual S, Pena CE, Porta C, Raoul J-, Reeves H, Ricci S, Riordan S, Ross P, Roth A, Ru??z del Arbol L, Rydzewska G, Sangiovanni A, Sangro B, Santoro A, Scher??bl J, Scheulen M, Schilsky M, Schmid R, Schwartz J, Schwartz M, Seitz J-, Shan M, Sherman M, Sol?? R, Spengler U, Stemmer S, Strickland A, Szawlowski A, Tabernero J, Takov D, Tchernev K, Tebbutt N, Thomson B, Tomczak P, Trevisani F, Van Laethem J-, Van Steenbergen W, Van Vlierberghe H, Wiest R, Zarba JJ, Zeuzem S
Clin. Cancer Res. 2012 18:2290-300
Species: Human
Sample Type: Plasma


Local cytokine concentrations and oxygen pressure are related to maturation of the collateral circulation in humans.
Baan J, Fledderus JO, Henriques JP, Hoefer IE, Horrevoets AJ, Koch KT, Moerland PD, Piek JJ, Schirmer SH, van der Schaaf RJ, van Royen N, Vis MM
J. Am. Coll. Cardiol. 2009 53:2141-7
Species: Human
Sample Type: Plasma


Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates.
Gore L, Kanter J, Kelher M, Khan SY, Silliman CC
Clin. Cancer Res. 2008 14:3942-7
Species: Human
Sample Type: Platelet-Poor Plasma


Fibroblast growth factor-2 expression is altered in lambs with increased pulmonary blood flow and pulmonary hypertension.
Azakie A, Benavidez E, Black SM, Devol JM, Fineman JR, Grobe A, Wedgwood S
Pediatr. Res. 2007 61:32-6
Species: Ovine
Sample Type: Serum


Serum concentrations of angiogenic cytokines in Waldenstrom macroglobulinaemia: the ration of angiopoietin-1 to angiopoietin-2 and angiogenin correlate with disease severity.
Anagnostopoulos A, Bamias A, Dimopoulos MA, Eleftherakis-Papaiakovou V, Kastritis E, Meletis J, Terpos E, Tsionos K
Br. J. Haematol. 2007 137:560-8
Species: Human
Sample Type: Serum


Endostatin variations in childhood acute lymphoblastic leukaemia--comparison with basic fibroblast growth factor and vascular endothelial growth factor.
Boquet C, Cazin L, Corbiere C, Legrand E, Marie-Cardine A, Schneider P, Vannier JP, Vasse M
Leuk. Res. 2007 31:629-38
Species: Human
Sample Type: Plasma


Dedicated epithelial recipient cells determine pigmentation patterns.
Brissette JL, Grossi M, Han R, Hasegawa K, Lee D, Li J, Scicchitano BM, Weiner L
Cell 2007 130:932-42
Species: Mouse
Sample Type: Cell Culture Supernates


Elevated levels of fibroblast growth factor-2 in serum from women with endometriosis.
Bourlev V, Larsson A, Olovsson M
Am. J. Obstet. Gynecol. 2006 194:755-9
Species: Human
Sample Type: Peritoneal Fluid


Phase II study of recombinant human endostatin in patients with advanced neuroendocrine tumors.
Bergsland EK, Enzinger PC, Fogler WE, Fuchs CS, Heymach JV, Kinsella K, Kulke MH, Lynch TJ, Meyerhardt JA, Michelini A, Ryan DP, Sidor C, Venook AP, Zhu AX
J. Clin. Oncol. 2006 24:3555-61
Species: Human
Sample Type: Urine


Phase I clinical and pharmacokinetic study of PTK/ZK, a multiple VEGF receptor inhibitor, in patients with liver metastases from solid tumours.
Drevs J, Gunther C, Hennig J, Ho YY, Laurent D, Lebwohl D, Marme D, Masson E, Medinger M, Morgan B, Mross K, Muller M, Unger C
Eur. J. Cancer 2005 41:1291-9
Species: Human
Sample Type: Plasma