Human IL-10 Quantikine HS ELISA Kit | hIL-10 QKit HS Kit
掲載日情報：2016/08/18 現在Webページ番号：111234
R&D Systems製のHuman IL-10 Quantikine HS ELISA Kitを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月08日 00時01分現在]
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IL-10, Human, ELISA Kit, Quantikine HS (96well) |
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[在庫・価格 ：2024年05月08日 00時01分現在]
IL-10, Human, ELISA Kit, Quantikine HS (96well)
文献数： 44
- 商品コード：HS100C
- メーカー：RSD
- 包装：1kit
- 価格：¥124,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物,カルタヘナ該当品
| 説明文 |
測定範囲：0.78 - 50 pg/mL，感度：0.17 pg/mL，測定試料：Serum (200 uL), EDTA Plasma (200 uL), Citrate Plasma (200 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，別売品コントロール：#QC41，測定時間：5.5 hours，測定因子：IL-10 別名：CSIF Genbank No： 3586 |
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| 法規制等 | 医薬用外毒物,カルタヘナ該当品 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
新型コロナウイルス感染に起因するサイトカインストームのモニタリング用製品 Helper T Cells Marker Antibodies |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5.5 hours
- Sample Type & Volume Required Per Well
- Serum (200 µL), EDTA Plasma (200 µL), Citrate Plasma (200 µL)
- Sensitivity
- 0.17 pg/mL
- Assay Range
- 0.78 - 50 pg/mL (Serum, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-10
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine HS Human IL-10 Immunoassay is a 5.5 hour solid phase ELISA designed to measure IL-10 in serum and plasma. It contains Sf 21-expressed recombinant human IL-10 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-10. Results obtained using natural human IL-10 showed linear curves that are parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-10.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 35 | 35 | 35 |
| Mean | 2.36 | 10.7 | 21 | 1.68 | 10.4 | 20.9 |
| Standard Deviation | 0.22 | 0.62 | 0.97 | 0.22 | 0.81 | 1.78 |
| CV% | 9.4 | 5.8 | 4.6 | 12.8 | 7.8 | 8.5 |
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Recovery
The recovery of IL-10 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 100 | 94-108 |
| Citrate Plasma (n=4) | 93 | 81-106 |
| EDTA Plasma (n=4) | 90 | 84-99 |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of IL-10 in various matrices and diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IL-10
- Aliases
- CSIF
- Entrez Gene IDs
- 3586 (Human); 16153 (Mouse); 25325 (Rat); 397106 (Porcine); 403628 (Canine); 493683 (Feline); 100135553 (Guinea Pig); 2949786 (Viral)
- Background
- IL-10
- Interleukin 10 (IL-10), initially designated cytokine synthesis inhibitory factor (CSIF), was originally identified as a product of mouse T helper (Th) 2 clones that inhibited cytokine production by Th1 clones . The human ortholog of mouse IL-10 was subsequently cloned by cross-hybridization. Human and mouse IL-10 are 81% identical at the nucleotide and amino acid level. IL-10 is the prototypic member of the IL-10 cytokine family, including IL-10, IL-19, IL-20, IL-22 (IL-TIF), IL-24 and IL-26. A number of viruses, including Epstein-Barr virus (EBV) and human cytomegalovirus (HCMV), also encode viral members of the IL-10 family. Human IL-10 has cross-species actvity and is active on mouse cells. Mouse IL-10 is species-specific and does not act on human cells.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Whole-Body UVB Irradiation during Allogeneic Hematopoietic Cell Transplantation Is Safe and Decreases Acute Graft-versus-Host Disease.
Hoffmann P, Karrer S, Kreutz M
J. Invest. Dermatol. 2012 132:179-87
Species: Human
Sample Type: Serum
Time-course of changes in inflammatory response after whole-body cryotherapy multi exposures following severe exercise.
Barbiche E, Bieuzen F, Fillard JR, Hausswirth C, Louis J, Pournot H
PLoS ONE 2011 6:e22748
Species: Human
Sample Type: Plasma
Inflammation associated with obesity: relationship with blood and bone marrow endothelial cells.
Berger AM, Bilek LD, Brusnahan SK, Garvin KL, Jackson JD, Kessinger MA, McGuire TR, O'Kane BJ, Sharp JG, Tuljapurkar SR
Obesity (Silver Spring) 2011 19:2130-6
Species: Human
Sample Type: Plasma
SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
Auwerx J, Elliott P, Itkonen P, Kainulainen S, Laakso M, Lagouge M, Modi S, Pihlajamaki J, Rutanen J, Sinclair DA, Vanttinen M, Westphal C, Yaluri N, Yamamoto H
Diabetes 2010 59:829-35
Species: Human
Sample Type: Plasma
Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
Laakso M, Pihlajamaki J, Punnonen K, Ruotsalainen E, Salmenniemi U, Stancakova A, Vauhkonen I
Atherosclerosis 2010 210:536-41
Species: Human
Sample Type: Plasma
Interleukin-18 is a strong predictor of cardiovascular events in elderly men with the metabolic syndrome: synergistic effect of inflammation and hyperglycemia.
Arnesen H, Hjerkinn EM, Seljeflot I, Troseid M
Diabetes Care 2009 32:486-92
Species: Human
Sample Type: Serum
Two large preoperative doses of erythropoietin do not reduce the systemic inflammatory response to cardiac surgery.
Andersen LW, Gotze JP, Jorgensen OS, Olsen NV, Poulsen TD, Steinbruchel D
J. Cardiothorac. Vasc. Anesth. 2009 23:316-23
Species: Human
Sample Type: Plasma
Age-dependent mobilization of circulating endothelial progenitor cells in infants and young children undergoing cardiac surgery with cardiopulmonary bypass.
Gu C, Liu Y, Pei J, Ren J, Sun G, Sun Y, Wang J, Wang Y, Yi D, Zhang S, Zheng R
Cytokine 2009 47:206-13
Species: Human
Sample Type: Plasma
Urinary proinflammatory cytokine response in renal transplant recipients with polyomavirus BK viruria.
Daniel V, Lahdou I, Naujokat C, Opelz G, Sadeghi M, Schnitzler P, Zeier M
Transplantation 2009 88:1109-16
Species: Human
Sample Type: Urine
Angiopoietin-2, marker and mediator of endothelial activation with prognostic significance early after trauma?
Bir ND, Brohi K, Chesebro BB, Christiaans SC, Cohen MJ, Ganter MT, Pittet JF, Rahn P, Staudenmayer KL
Ann. Surg. 2008 247:320-6
Species: Human
Sample Type: Plasma
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