Human XCL1/Lymphotactin Quantikine ELISA Kit | hXCL1/Lymphotactin QKit
掲載日情報：2016/08/18 現在Webページ番号：111233
R&D Systems製のHuman XCL1/Lymphotactinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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Human XCL1/Lymphotactin Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月26日 20時55分現在]
Human XCL1/Lymphotactin Quantikine ELISA Kit
文献数： 0
- 商品コード：DXCL10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ヒト試料中に存在する，Lymphotactin (XCL1)をサンドイッチ法により比色定量するELISAキットです。測定試料：Serum, Plasma, Cell Culture Supernatant，測定範囲：0.156-10 ng/mL，測定波長：450 nm，別売品コントロール：#QC117，種交差性：Human，アッセイ法：Solid Phase Sandwich ELISA，感度：0.023 ng/mL，測定時間：4.5 hours，測定因子：XCL1, Lymphotactin 別名：ATAC Genbank No： 6375 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Quantikine Human Lymphotactin ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 0.023 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human Lymphotactin. This assay also recognizes human XCL2.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human XCL1/Lymphotactin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lymphotactin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Lymphotactin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lymphotactin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lymphotactin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.92 | 2.83 | 5.57 | 1.01 | 3.08 | 6.29 |
| Standard Deviation | 0.024 | 0.078 | 0.116 | 0.095 | 0.278 | 0.494 |
| CV% | 2.6 | 2.8 | 2.1 | 9.4 | 9 | 7.9 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.12 | 3.27 | 6.66 | 1.14 | 3.32 | 6.69 |
| Standard Deviation | 0.031 | 0.085 | 0.184 | 0.084 | 0.199 | 0.374 |
| CV% | 2.8 | 2.6 | 2.8 | 7.4 | 6 | 5.6 |
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Recovery
The recovery of human Lymphotactin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 109 | 99-116 |
| Serum (n=4) | 98 | 89-107 |
| EDTA Plasma (n=4) | 95 | 86-100 |
| Heparin Plasma (n=4) | 93 | 87-101 |
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Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human Lymphotactin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- XCL1/Lymphotactin
- Aliases
- ATAC; Lymphotactin; SCM-1 alpha
- Entrez Gene IDs
- 6375 (Human); 16963 (Mouse)
- Background
- XCL1/Lymphotactin
- Lymphotactin (Lptn), also known as SCM-1a and ATAC, belongs to the C or gamma subfamily of chemokines and has been designated XCL1. Human Lymphotactin encodes a 114 amino acid (aa) precursor with a 21 aa predicted signal peptide. The gene for lymphotactin has been mapped to chromosome 1 in both human and mouse.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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