Human VEGF-C Quantikine ELISA Kit | hVEGF-C QKit
掲載日情報：2016/08/18 現在Webページ番号：111228
R&D Systems製のHuman VEGF-Cを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月05日 00時00分現在]
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VEGF-C, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月05日 00時00分現在]
VEGF-C, Human, ELISA Kit, Quantikine (96well)
文献数： 53
- 商品コード：DVEC00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：109.00 - 7000 pg/mL，感度：48.4 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (50 uL), Platelet-poor Plasma (50 uL), Heparin Plasma (50 uL), Saliva (50 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC24，測定時間：4.5 hours，測定因子：VEGF-C 別名：Flt4 ligand Genbank No： 7424 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2019年12月15日号 p.3 ニュース2018年3月1日号 p.21
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| 製品記事 |
R&D Systems社 VEGF-VEGF R2シグナル伝達系関連製品 |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (50 µL), Platelet-poor Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL)
- Sensitivity
- 48.4 pg/mL
- Assay Range
- 109 - 7,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma, Platelet-poor Plasma)
- Specificity
- Natural human VEGF-C, recombinant human VEGF-C (wild type), and recombinant human VEGF-C (Cys156Ser substituted)
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human VEGF-C Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human VEGF-C in cell culture supernates, serum, plasma, and saliva. It contains NS0-expressed recombinant human VEGF-C (Cys156Ser substituted) and has been shown to accurately quantitate the recombinant factor. Results obtained using recombinant human VEGF-C (wild type) and natural human VEGF-C showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human VEGF-C.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Platelet-poor Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 512 | 1,543 | 3,195 | 514 | 1,540 | 3,066 |
| Standard Deviation | 33.8 | 54.2 | 136 | 43.6 | 110 | 198 |
| CV% | 6.6 | 3.5 | 4.3 | 8.5 | 7.2 | 6.4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 572 | 1,622 | 3,243 | 520 | 1,527 | 3,069 |
| Standard Deviation | 39.3 | 54.4 | 136 | 50.1 | 102 | 200 |
| CV% | 6.9 | 3.4 | 4.2 | 9.6 | 6.7 | 6.5 |
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Recovery
The recovery of VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 99 | 89-106 |
| Heparin Plasma (n=4) | 97 | 88-103 |
| Cell Culture Supernates (n=4) | 107 | 93-115 |
| EDTA Plasma (n=4) | 101 | 96-110 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of VEGF-C were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- VEGF-C
- Aliases
- undefined
- Entrez Gene IDs
- 7424 (Human); 22341 (Mouse)
- Background
- VEGF-C
- Vascular endothelial growth factor C (VEGF-C) and VEGF-D constitute a VEGF subfamily that shares the conserved VEGF homology domain (VHD) with other VEGF family members, but is distinguished by their preferential formation of non-covalent homodimers. VEGF-C (Cys156Ser) is a selective agonist of VEGF R3 and does not bind VEGF R2 (Joukov, V. et. al., 1998, J. Biol. Chem. 273:6599).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Co-expression of alpha9beta1 integrin and VEGF-D confers lymphatic metastatic ability to a human breast cancer cell line MDA-MB-468LN.
Bhattacharjee RN, Chambers AF, Lala PK, Majumder M, Rodriguez-Torres M, Timoshenko AV, Torres-Garcia J, Tutunea-Fatan E, Wiebe R, Xin X
PLoS ONE 2012 7:e35094
Species: Human
Sample Type: Cell Culture Supernates
Lysophosphatidic Acid Enhances Vascular Endothelial Growth Factor-C Expression in Human Prostate Cancer PC-3 Cells.
Chen SU, Lin CC, Lin CE
PLoS ONE 2012 7:e41096
Species: Human
Sample Type: Cell Culture Supernates
Proteinuria triggers renal lymphangiogenesis prior to the development of interstitial fibrosis.
de Borst M, Hovingh M, Kramer A, Kwakernaak A, Mirkovic K, Navis G, Poosti F, Sjollema K, Slagman M, van den Born J, van Goor H, Yazdani S
PLoS ONE 2012 7:e50209
Species: Human
Sample Type: Cell Culture Supernates
Mechanism-related circulating proteins as biomarkers for clinical outcome in patients with unresectable hepatocellular carcinoma receiving sunitinib.
Boucher E, Cheng AL, DePrimo SE, Douillard JY, Faivre S, Harmon CS, Kim JS, Lanzalone S, Lechuga MJ, Lim HY, Lin X, Raymond E
J Transl Med 2011 9:120
Species: Human
Sample Type: Plasma
Prognostic significance of angiogenic factors in uterine cervical cancer.
Blohmer JU, Heidecke H, Jeschke S, Korlach S, Kummel S, Landt S, Lichtenegger W, Schmid P, Sehouli J, Stoblen F, Wehling M
Anticancer Res. 2011 31:2589-95
Species: Human
Sample Type: Serum
Human pregnancy specific beta-1-glycoprotein 1 (PSG1) has a potential role in placental vascular morphogenesis.
Dizon AM, Dveksler GS, Ergun S, Ha CT, Irmak S, Lisboa FA, Warren JW, Wu JA
Biol. Reprod. 2010 83:27-35
Species: Human
Sample Type: Cell Culture Supernates
Phase I and pharmacological study of the broad-spectrum tyrosine kinase inhibitor JNJ-26483327 in patients with advanced solid tumours.
Burger H, de Jonge MJ, Konings IR
Br. J. Cancer 2010 103:987-92
Species: Human
Sample Type: Serum
Macrophages regulate salt-dependent volume and blood pressure by a vascular endothelial growth factor-C-dependent buffering mechanism.
Alitalo K, Beck FX, Dahlmann A, Derer W, Dietsch P, Eckardt KU, Goss J, Hilgers KF, Jantsch J, Kerjaschki D, Kurtz A, Luft FC, Machnik A, Machura K, Muller DN, Neuhofer W, Park JK, Tammela T, Titze J, Van Rooijen N, Wagner H, Ziomber A
Nat. Med. 2009 15:545-52
Species: Human
Sample Type: Serum
Efficacy, safety, and potential biomarkers of sunitinib monotherapy in advanced hepatocellular carcinoma: a phase II study.
Abrams TA, Ancukiewicz M, Bhargava P, Blaszkowsky LS, Catalano OA, Clark JW, di Tomaso E, Duda DG, Enzinger PC, Fuchs CS, Hezel AF, Jain RK, Kwak EL, Lahdenranta J, Meyerhardt J, Miksad R, Ryan DP, Sahani DV, Sindhwani V, Yoon SS, Zhu AX
J. Clin. Oncol. 2009 27:3027-35
Species: Human
Sample Type: Plasma
VEGF-C regulates lymphangiogenesis and capillary stability by regulation of PDGF-B.
Fujii T, Hasegawa M, Kohno R, Nakagawa K, Nakano T, Nishikawa S, Onimaru M, Sueishi K, Tanii M, Yonemitsu Y
Am. J. Physiol. Heart Circ. Physiol. 2009 297:H1685-96
Species: Human
Sample Type: Serum
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