Human u-Plasminogen Activator/Urokinase Quantikine ELISA | huPA Qkit
掲載日情報：2016/08/18 現在Webページ番号：111223
R&D Systems製のHuman u-Plasminogen Activator/Urokinase Quantikine ELISAを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
u-Plasminogen Activator, Human, ELISA Kit, Quantikine (96well) <Urokinase, ELISA Kit> <uPA, ELISA Kit> |
|
3 | |||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月26日 20時55分現在]
u-Plasminogen Activator, Human, ELISA Kit, Quantikine (96well) <Urokinase, ELISA Kit> <uPA, ELISA Kit>
文献数： 3
- 商品コード：DUPA00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 種交差性：Human，感度：4.17 pg/mL，測定範囲：31.3-2,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Tissue Lysate，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC180，測定時間：4.5 hours，測定因子：u-Plasminogen Activator (uPA), Urokinase 別名：ATF Genbank No： 5328 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
|
||
| 製品記事 | Quantikine Human u-Plasminogen Activator ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
||
| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
||
追加しました。
Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (17 µL), EDTA Plasma (17 µL), Heparin Plasma (17 µL), Urine (10 µL)
- Sensitivity
- 4.17 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human uPA. This assay also recognizes uPA complexed with PAI-1 or uPAR.
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Human u-Plasminogen Activator/Urokinase Immunoassay is a 4.5 hour solid phase ELISA designed to measure uPA in cell culture supernates, cell lysates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human uPA. Results obtained using natural human uPA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human uPA.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 230 | 629 | 1,219 | 214 | 614 | 1,188 |
| Standard Deviation | 4.8 | 8.9 | 29.3 | 15.1 | 39.8 | 82 |
| CV% | 2.1 | 1.4 | 2.4 | 7.1 | 6.5 | 6.9 |
追加しました。
Recovery
The recovery of uPA spiked to three different levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=8) | 102 | 93-115 |
| Cell Lysates (n=4) | 97 | 81-116 |
| Serum (n=4) | 87 | 81-93 |
| EDTA Plasma (n=4) | 87 | 83-94 |
| Heparin Plasma (n=4) | 89 | 81-110 |
| Urine (n=4) | 98 | 81-119 |
追加しました。
Linearity
To assess the linearity of the assay, samples containing high concentrations of uPA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
追加しました。
Additional Infomation
- Molecule Information
- u-Plasminogen Activator/Urokinase
- Aliases
- PLAU; uPA; Urokinase
- Entrez Gene IDs
- 5328 (Human)
- Background
- uPA
- u-Plasminogen Activator (uPA) is a serine protease that converts plasminogen to plasmin, with roles in a variety of normal and pathological processes thatinclude cell migration and tissue destruction. uPA is a potent marker of invasion and metastasis in a variety of human cancers including breast, stomach, colon, bladder, ovarian, brain, and endometrium.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。