Human uPAR Quantikine ELISA Kit | huPAR Q Kit
掲載日情報：2016/08/18 現在Webページ番号：111222
R&D Systems製のHuman uPARを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 10時55分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
uPA Receptor, Human, ELISA Kit, Quantikine (96well) |
|
39 | |||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月29日 10時55分現在]
uPA Receptor, Human, ELISA Kit, Quantikine (96well)
文献数： 39
- 商品コード：DUP00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：62.5-4,000 pg/mL，感度：33 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC21，測定時間：4.5 hours，測定因子：uPAR 別名：CD87 Genbank No： 5329 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2017年11月1日号 p.17
|
||
| 製品記事 | uPAレセプター研究用製品 ELISA Kit製品に関するFAQ（R&D Systems） |
||
| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
- Sensitivity
- 33 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human uPAR
- Interference
- Interference observed with 1 or more available related molecules.
追加しました。
Product Summary
The Quantikine Human uPAR Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human uPAR in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human uPAR and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant factor. Results obtained using natural human uPAR showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human uPAR.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 836 | 1,593 | 2,412 | 796 | 1,546 | 2,300 |
| Standard Deviation | 17.3 | 65.4 | 181 | 44.3 | 78.9 | 136 |
| CV% | 2.1 | 4.1 | 7.5 | 5.6 | 5.1 | 5.9 |
追加しました。
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of uPAR were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
追加しました。
Additional Infomation
- Molecule Information
- uPAR
- Aliases
- PLAUR
- Entrez Gene IDs
- 5329 (Human); 18793 (Mouse)
- Background
- uPAR
- The urokinase-type plasminogen activator (uPA) is one of two activators that converts plasminogen to plasmin. uPA binds with high-affinity to the cell surface via the GPI-linked receptor uPAR, which serves to localize uPA proteolytic activity. Human uPAR cDNA encodes a 335 amino acid (aa) precursor protein with a 22 aa signal peptide, five potential N-linked glycosylation sites and a C-terminal GPI-anchor site.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Serum-soluble urokinase receptor concentration in primary FSGS.
Deegens JK, Maas RJ, Wetzels JF
Kidney Int. 2012 81:1043-4
Species: Human
Sample Type: Serum
Circulating urokinase receptor as a cause of focal segmental glomerulosclerosis.
Burke G, Chaudhuri A, Daftarian P, El Hindi S, Fornoni A, Goes N, Gupta V, Karumanchi SA, Li J, Maiguel D, Morath C, Nikolic B, Rastaldi MP, Reiser J, Roth D, Ruiz P, Sageshima J, Saleem M, Salido E, Salifu M, Sarwal MM, Schaefer F, Schwenger V, Torres A, Wei C, Yap HK, Zeier M, Zhang Q
Nat. Med. 2011 17:952-60
Species: Human
Sample Type: Serum
Identification of regulatory Hck and PAI-2 proteins in the monocyte response to PEG-containing matrices.
Brown JF, Kao WJ, Zuckerman ST
Biomaterials 2009 30:3825-33
Species: Human
Sample Type: Cell Culture Supernates
The human airway trypsin-like protease modulates the urokinase receptor (uPAR, CD87) structure and functions.
Beaufort N, Chignard M, Eguchi H, Fend F, Hellmann D, Kamimura T, Leduc D, Masegi T, Mengele K, Pidard D, Yasuoka S
Am. J. Physiol. Lung Cell Mol. Physiol. 2007 292:L1263-72
Species: Human
Sample Type: Cell Culture Supernates
Urokinase-type plasminogen activator modulates airway eosinophil adhesion in asthma.
Bates ME, Bertics PJ, Brooks AM, Jarjour NN, Sedgwick JB, Vrtis RF
Am. J. Respir. Cell Mol. Biol. 2006 35:503-11
Species: Human
Sample Type: BALF
Low oxygen concentrations inhibit trophoblast cell invasion from early gestation placental explants via alterations in levels of the urokinase plasminogen activator system.
Bulmer JN, Innes BA, Lash GE, Otun HA, Robson SC, Searle RF
Biol. Reprod. 2005 74:403-9
Species: Human
Sample Type: Cell Culture Supernates
The fibroblast growth factor receptor-4 Arg388 allele is associated with prostate cancer initiation and progression.
Ittmann M, Stockton DW, Wang J
Clin. Cancer Res. 2004 10:6169-78
Species: Human
Sample Type: Cell Lysates
Soluble urokinase-type plasminogen activator receptor (suPAR) as an independent factor predicting worse prognosis and extra-bone marrow involvement in multiple myeloma patients.
Bragotti LZ, Carroccia R, Castagnari B, Castoldi G, Cavazzini F, Ciccone M, Cuneo A, Della Porta M, Guerra G, Rigolin GM, Tieghi A
Br. J. Haematol. 2003 120:953-9
Species: Human
Sample Type: Serum
Pretreatment determination of the serum urokinase plasminogen activator and its soluble receptor in advanced small-cell lung cancer or non-small-cell lung cancer.
Cobos E, Jumper C, Lox C
Clin. Appl. Thromb. Hemost. 2003 9:241-6
Species: Human
Sample Type: Serum
The circulating urokinase plasminogen activator (uPA) and its soluble receptor (suPAR) are not up-regulated by the circulating P105 fraction of the HER-2/neu proto-oncogene: in vivo evidence from patients with advanced non-small cell lung cancer (NSCLC).
Cobos E, Jumper C, Lox C
Anticancer Res. 2002 22:2073-6
Species: Human
Sample Type: Serum
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。