Human Thrombospondin-2 Quantikine ELISA Kit | hTSP-2 QKit
掲載日情報：2016/08/18 現在Webページ番号：111219
R&D Systems製のHuman Thrombospondin-2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時55分現在]
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Thrombospondin-2, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月29日 20時55分現在]
Thrombospondin-2, Human, ELISA Kit, Quantikine (96well)
文献数： 4
- 商品コード：DTSP20
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.31-20 ng/mL，感度：0.068 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Human Milk，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC171，測定時間：4.5 hours，測定因子：Thrombospondin-2 別名：THBS2 Genbank No： 7058 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Thrombospondin-2 ELISA Kit（R&D Systems社） ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL), Human Milk (50 µL)
- Sensitivity
- 0.068 ng/mL
- Assay Range
- 0.31 - 20 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, Human Milk, EDTA Plasma)
- Specificity
- Natural and recombinant human Thrombospondin-2
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Thrombospondin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Thrombospondin-2 in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human Thrombospondin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Thrombospondin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Thrombospondin-2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 3.97 | 7.96 | 11.4 | 4.12 | 7.68 | 11.4 |
| Standard Deviation | 0.198 | 0.205 | 0.284 | 0.259 | 0.403 | 0.527 |
| CV% | 5 | 2.6 | 2.5 | 6.3 | 5.2 | 4.6 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 4.08 | 7.73 | 10.9 | 4.11 | 7.71 | 11.4 |
| Standard Deviation | 0.189 | 0.229 | 0.281 | 0.295 | 0.511 | 0.726 |
| CV% | 4.6 | 3 | 2.6 | 7.2 | 6.6 | 6.4 |
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Recovery
The recovery of Thrombospondin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 93 | 86-105 |
| Heparin Plasma (n=4) | 92 | 86-103 |
| EDTA Plasma (n=4) | 93 | 86-105 |
| Cell Culture Media (n=4) | 101 | 91-108 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Thrombospondin-2 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Thrombospondin-2
- Aliases
- THBS2; TSP-2
- Entrez Gene IDs
- 7058 (Human)
- Background
- Thrombospondin-1, -2, and -4
- Thrombospondins (TSP) are secreted multidomain glycoproteins with many putative functions including modulating cell adhesion, proliferation, migration, and angiogenesis. At least five TSPs exist: TSP-1, -2, -3, -4, and cartilage oligomeric glycoprotein (COMP)/TSP-5. The family can be divided into two subgroups. The first, TSP-1 and -2, are homotrimeric molecules, each monomer containing an N-terminal/heparin-binding domain, a coiled-coil oligomerization domain, a von Willebrand factor-type/procollagen homology domain, three type 1 thrombospondin repeats (TSR), three EGF-like repeats, seven TSP type 3 repeats, and a C-terminal globular domain. TSP-1 and -2 are thought to mediate interactions between cells and the extracellular matrix, and as such are matricellular proteins. In contrast, TSP-3, -4, and COMP/TSP-5 are homopentamers that lack the N-terminal structure of TSP-1 and -2, but have a similar arrangement of EGF and TSP type 3 repeats, and the C-terminal domain characteristic of the TSP family.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Tumor-derived pancreatic stellate cells promote pancreatic cancer cell invasion through release of thrombospondin-2.
Berger DH, Farrow B, Rowley D
J. Surg. Res. 2009 156:155-60
Species: Human
Sample Type: Cell Culture Supernates
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