Human TFF3 Quantikine ELISA Kit | hTFF3 QKit
掲載日情報：2016/08/18 現在Webページ番号：111205
R&D Systems製のHuman TFF3を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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TFF3, Human, ELISA Kit, Quantikine |
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[在庫・価格 ：2025年04月26日 20時55分現在]
TFF3, Human, ELISA Kit, Quantikine
文献数： 5
- 商品コード：DTFF30
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：13.1 pg/mL，測定範囲：39-2,500 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Saliva，種交差性：Human，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC166，測定時間：4.5 hours，測定因子：TFF3 別名：HITF Genbank No： 7033 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human TFF3 Quantikine(R) ELISA Kit Quantikine Human TFF3 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)
- Sensitivity
- 13.1 pg/mL
- Assay Range
- 39 - 2,500 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Trefoil Factor 3 (TFF3)
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human TFF3 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human TFF3 in cell culture supernates, serum, plasma, saliva, and urine. It contains CHO cell-expressed recombinant human TFF3 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human TFF3 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human TFF3.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 4 | 4 | 4 | 4 | 4 | 4 |
| Mean | 276 | 849 | 1,684 | 286 | 894 | 1,712 |
| Standard Deviation | 6.01 | 9.13 | 37.4 | 18.3 | 44 | 95.9 |
| CV% | 2.2 | 1.1 | 2.2 | 6.4 | 4.9 | 5.6 |
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Recovery
The recovery of human TFF3 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Supernates (n=4) | 100 | 90-114 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of human TFF3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- TFF3
- Aliases
- ITF; TFI
- Entrez Gene IDs
- 7033 (Human); 21786 (Mouse); 25563 (Rat)
- Background
- TFF3
- TFF3 (Trefoil factor 3; also hP1.B and intestinal trefoil factor) is a 7 kDa member of the trefoil factor family of protease-resistant peptides. It is expressed by multiple epithelial cell types, acts in an autocrine or paracrine manner and contributes to epithelial cell migration and survival. TFF3 is secreted by mucus-producing goblet cells in the large and small intestines and lower respiratory tract.Mature human TFF3 is 59 amino acids (aa) in length (aa 22 - 80). It contains one P (or trefoil) domain that is characterized by the presence of three cysteine bond-mediated loops (aa 30 - 72). Human TFF3 exists as both a monomer and disulfide-linked dimer. There are three potential isoform variants. One shows an alternate start site at Met8, a second shows a 50 aa N-terminal extension and a third shows a 109 aa substitution for aa 29 - 80. Over aa 21 - 80, human TFF3 shares 77%, 68% and 72% aa identity with mouse, canine, and rat TFF3, respectively.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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