Human SPARC Quantikine ELISA Kit | hSPARC Qkit
掲載日情報：2016/08/18 現在Webページ番号：111198
R&D Systems製のHuman SPARCを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
| 詳細 | 商品名 |
|
文献数 | ||||||||||||||||||||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
|
Human SPARC Quantikine ELISA Kit |
|
9 | |||||||||||||||||||||||||||||||||||||||||||||||||||
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||
[在庫・価格 ：2025年04月27日 00時00分現在]
Human SPARC Quantikine ELISA Kit
文献数： 9
- 商品コード：DSP00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：1.56-50 ng/mL，感度：0.269 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Human Milk，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC160，測定時間：4.5 hours，測定因子：SPARC 別名：Basement-membrane protein 40 Genbank No： 6678 |
||
|---|---|---|---|
| 法規制等 | |||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2021年5月1日号 p.8
|
||
| 製品記事 | Quantikine Human SPARC ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
||
| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
||
追加しました。
Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (10 µL), Serum (10 µL), Platelet-poor EDTA Plasma (20 µL), Platelet-poor Heparin Plasma (20 µL), Human Milk (50 µL)
- Sensitivity
- 0.269 ng/mL
- Assay Range
- 1.56 - 50 ng/mL (Serum, Cell Culture Supernates, Human Milk, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant human SPARC
- Interference
- No significant interference observed with available related molecules.
追加しました。
Product Summary
The Quantikine Human SPARC Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SPARC in cell culture supernates, serum, plasma, and human milk. It contains NS0-expressed recombinant human SPARC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human SPARC showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human SPARC.
追加しました。
Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 7.34 | 14.3 | 29.9 | 6.65 | 13.5 | 28.3 |
| Standard Deviation | 0.129 | 0.282 | 0.733 | 0.541 | 0.989 | 2.4 |
| CV% | 1.8 | 2 | 2.5 | 8.1 | 7.3 | 8.5 |
追加しました。
Recovery
The recovery of SPARC spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 102 | 86-111 |
| Platelet-poor EDTA Plasma (n=4) | 104 | 93-111 |
| Platelet-poor Heparin Plasma (n=4) | 91 | 82-100 |
追加しました。
Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SPARC were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
追加しました。
Additional Infomation
- Molecule Information
- SPARC
- Aliases
- BM-40; Osteonectin
- Entrez Gene IDs
- 6678 (Human); 20692 (Mouse)
- Background
- SPARC
- SPARC, also known as Osteonectin and Basement Membrane protein BM-40, is a phosphorylated glycoprotein with calcium-binding domains. It is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. SPARC may be involved in cellproliferation, repair of tissue damage, and morphogenic processes such as re-modeling of the extracellular matrix.
追加しました。
Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
SPARC expression in CML is associated to imatinib treatment and to inhibition of leukemia cell proliferation.
Barbagallo I, Chiarenza A, Consoli C, Cupri A, Di Raimondo F, Giallongo C, La Cava P, Palumbo G, Parrinello N, Stagno F, Tibullo D
BMC Cancer 2013 13:60
Species: Human
Sample Type: Serum
追加しました。
製品情報は掲載時点のものですが、価格表内の価格については随時最新のものに更新されます。お問い合わせいただくタイミングにより製品情報・価格などは変更されている場合があります。
表示価格に、消費税等は含まれていません。一部価格が予告なく変更される場合がありますので、あらかじめご了承下さい。