Human sE-Selectin/CD62E Quantikine ELISA Kit | hsE-Selectin/CD62E Qkit
掲載日情報：2016/08/18 現在Webページ番号：111197
R&D Systems製のHuman sE-Selectin/CD62Eを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 10時55分現在]
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Human sE-Selectin/CD62E Quantikine ELISA Kit (1 KT) |
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[在庫・価格 ：2025年04月29日 10時55分現在]
Human sE-Selectin/CD62E Quantikine ELISA Kit (1 KT)
文献数： 42
- 商品コード：DSLE00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：0.027 ng/mL，測定範囲：0.125-8 ng/mL，種交差性：Human，測定試料：Serum, Plasma, Cell Culture Supernatant，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC85，測定時間：4.5 hours，測定因子：E-Selectin, CD62E 別名：CD62 antigen-like family member E Genbank No： 6401 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | R&D Systems社 E-Selectin関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
- Sensitivity
- 0.027 ng/mL
- Assay Range
- 0.125 - 8 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma)
- Specificity
- Natural and recombinant human sE-Selectin
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human sE-Selectin immunoassay is a 4.5 hour solid phase ELISA designed to measure sE-Selectin in cell culture supernates, serum, and plasma. It contains CHO cell-expressed recombinant human sE-Selectin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human sE-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human sE-Selectin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.6 | 2.9 | 4.7 | 1.5 | 2.9 | 4.6 |
| Standard Deviation | 0.11 | 0.17 | 0.24 | 0.11 | 0.25 | 0.35 |
| CV% | 6.6 | 5.7 | 5.2 | 7.3 | 8.7 | 7.7 |
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Recovery
The recovery of sE-Selectin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 96 | 85-106 |
| Heparin Plasma (n=4) | 94 | 85-107 |
| Citrate Plasma (n=4) | 96 | 85-107 |
| Cell Culture Media (n=4) | 103 | 98-110 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of sE-Selectin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- E-Selectin/CD62E
- Aliases
- CD62E; ELAM1; LECAM2; SELE
- Entrez Gene IDs
- 6401 (Human); 20339 (Mouse); 25544 (Rat)
- Background
- Selectins
- The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Endogenous granulocyte colony-stimulating factor: a biomarker in acute ischemic stroke.
Chang CS, Huang CS, Kuo CL, Liu CS, Su SL, Wu SL, Yu SC
Biomarkers 2012 17:319-24
Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
BMC Infect Dis 2012 12:144
Adipocytokine levels mark endothelial function in normotensive individuals.
Bruno R, Duranti E, Ghiadoni L, Santini E, Solini A, Stea F, Taddei S
Cardiovasc Diabetol 2012 11:103
Species: Human
Sample Type: Plasma
SIRT1 mRNA expression may be associated with energy expenditure and insulin sensitivity.
Auwerx J, Elliott P, Itkonen P, Kainulainen S, Laakso M, Lagouge M, Modi S, Pihlajamaki J, Rutanen J, Sinclair DA, Vanttinen M, Westphal C, Yaluri N, Yamamoto H
Diabetes 2010 59:829-35
Species: Human
Sample Type: Serum
Effect of intensive lifestyle changes on endothelial function and on inflammatory markers of atherosclerosis.
Beto RJ, Bhardwaj R, Dod HS, Gharib W, Hobbs GR, Jain AC, Konat GW, Manivannan S, Nanda NC, Ornish D, Sajja V, Warden BE, Weidner G
Am. J. Cardiol. 2010 105:362-7
Species: Human
Sample Type: Serum
Changes in cytokine levels during acute hyperinsulinemia in offspring of type 2 diabetic subjects.
Laakso M, Pihlajamaki J, Punnonen K, Ruotsalainen E, Salmenniemi U, Stancakova A, Vauhkonen I
Atherosclerosis 2010 210:536-41
Species: Human
Sample Type: Plasma
Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
Barr RG, Johnson WC, Kawut SM
Biomarkers 2010 15:731-8
Species: Human
Sample Type: Plasma
Prehospital resuscitation with hypertonic saline-dextran modulates inflammatory, coagulation and endothelial activation marker profiles in severe traumatic brain injured patients.
Baker AJ, Crnko NT, Rhind SG
J Neuroinflammation 2010 7:5
Species: Human
Sample Type: Serum
First-trimester prediction of gestational diabetes mellitus: examining the potential of combining maternal characteristics and laboratory measures.
Makgoba M, Messow CM, Nelson SM, Nicolaides K, Sattar N, Savvidou M
Diabetes 2010 59:3017-22
Species: Human
Sample Type: Serum
Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
Arnao V, Corrao S, Di Raimondo D, Di Sciacca R, Fernandez P, Licata G, Pinto A, Tuttolomondo A
Atherosclerosis 2009 203:503-8
Species: Human
Sample Type: Plasma
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