Human Serpin E1/PAI-1 Quantikine ELISA Kit | hSerpin E1/PAI-1 QKit
掲載日情報：2016/08/18 現在Webページ番号：111194
R&D Systems製のHuman Serpin E1/PAI-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月25日 00時00分現在]
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Serpin E1/PAI-1, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月25日 00時00分現在]
Serpin E1/PAI-1, Human, ELISA Kit, Quantikine (96well)
文献数： 32
- 商品コード：DSE100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.31 - 20 ng/mL，感度：0.142 ng/mL，測定試料：Cell Culture Supernates (50 uL), Cell Lysates (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL), Citrate Plasma (50 uL), CTAD Plasma (50 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC157，測定時間：4.5 hours，測定因子：Serpin E1/PAI-1 別名：Endothelial plasminogen activator inhibitor Genbank No： 5054 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Human / Total Human Serpin E1 (PAI-1) ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Citrate Plasma (50 µL), CTAD Plasma (50 µL)
- Sensitivity
- 0.142 ng/mL
- Assay Range
- 0.312 - 20 ng/mL (Heparin Plasma, Cell Culture Supernates, Cell Lysates, Citrate Plasma, EDTA Plasma, CTAD Plasma)
- Specificity
- Natural and recombinant human Serpin E1
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human Serpin E1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin E1 in cell culture supernates, cell lysates, and plasma. It contains Sf 21-expressed recombinant human Serpin E1 and has been shown to accurately quantitate the recombinant factor in its active and latent forms as well as in the vitronectin complexes. However, the kit does not detect recombinant human Serpin E1 in complexes with recombinant human uPA and recombinant human tPA. Results obtained using natural human Serpin E1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin E1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Citrate Plasma, CTAD Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 2.6 | 6.5 | 12.6 | 2.3 | 6.4 | 13.1 |
| Standard Deviation | 0.12 | 0.52 | 0.98 | 0.2 | 0.4 | 0.8 |
| CV% | 4.4 | 8 | 7.8 | 9.5 | 6.4 | 6.1 |
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Recovery
The recovery of Serpin E1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Heparin Plasma (n=4) | 98 | 85-113 |
| Citrate Plasma (n=4) | 99 | 88-112 |
| EDTA Plasma (n=4) | 100 | 85-115 |
| CTAD Plasma (n=4) | 100 | 83-112 |
| Cell Culture Media (n=4) | 106 | 89-113 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Serpin E1 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Serpin E1/PAI-1
- Aliases
- Nexin; PAI-1; PLANH1
- Entrez Gene IDs
- 5054 (Human); 18787 (Mouse)
- Background
- Serpins
- The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Dipyridamole decreases inflammatory metalloproteinase-9 expression and release by human monocytes.
Thromb Haemost 2013 109:280-9
Species: Human
Sample Type: Cell Culture Supernates
Recombinant GPI-anchored TIMP-1 stimulates growth and migration of peritoneal mesothelial cells.
Djafarzadeh R, Goyal P, Himmelein S, Nelson PJ, Noessner E, Notohamiprodjo S, Ribeiro A, Sauter M, Siess W, Sitter T, Wornle M
PLoS ONE 2012 7:e33963
Species: Human
Sample Type: Cell Lysates
Systemic biomarkers of inflammation and haemostasis in patients with chronic necrotizing pulmonary aspergillosis.
BMC Infect Dis 2012 12:144
Species: Human
Sample Type: Plasma
Endothelial differentiation of adipose-derived stem cells from elderly patients with cardiovascular disease.
Dimatteo C, DiMuzio PJ, Hager E, McIlhenny S, Moudgill N, Tarola N, Tulenko T, Zhang P
Stem Cells Dev. 2011 20:977-88
Species: Human
Sample Type: Cell Culture Supernates
Exercise training improves sleep pattern and metabolic profile in elderly people in a time-dependent manner.
Boscolo RA, Damaso AR, de Mello MT, Esteves AM, Grassmann V, Lira FS, Oller do Nascimento CM, Oyama LM, Pimentel GD, Santana MG, Santos RV, Tufik S, Viana VA
Lipids Health Dis 2011 10:113
Species: Human
Sample Type: Plasma
SPARC suppresses apoptosis of idiopathic pulmonary fibrosis fibroblasts through constitutive activation of beta-catenin.
Chang W, Jacobs SS, Rosen GD, Upadhyay D, Wei K, Weill D
J. Biol. Chem. 2010 285:8196-206
Species: Human
Sample Type: Cell Culture Supernates
Placenta growth factor (PlGF), a novel inducer of plasminogen activator inhibitor-1 (PAI-1) in sickle cell disease (SCD).
Patel N, Sundaram N, Yang M
J. Biol. Chem. 2010 285:16713-22
Species: Human
Sample Type: Plasma
Cyclic adenosine monophosphate induces plasminogen activator inhibitor-1 expression in human mast cells.
Kwong KY, Ma Z, Paek D, Tovar JP
Biochem. Biophys. Res. Commun. 2010 400:569-74
Species: Human
Sample Type: Cell Culture Supernates
Human CD4- 8- T cells are a distinctive immunoregulatory subset.
Goetzl EJ, Huang MC, Longo DL, Patel K, Taub DD
FASEB J. 2010 24:2558-66
Species: Human
Sample Type: Cell Culture Supernates
Syndromic and non-syndromic aneurysms of the human ascending aorta share activation of the Smad2 pathway.
Al Haj Zen A, Borges LF, Gomez D, Gutierrez PS, Jondeau G, Michel JB, Philippe M, Vranckx R
J. Pathol. 2009 218:131-42
Species: Human
Sample Type: Cell Culture Supernates
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