Human Serpin A12 Quantikine ELISA Kit | hSerpin A12 QKit
掲載日情報：2016/08/18 現在Webページ番号：111191
R&D Systems製のHuman Serpin A12を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月04日 00時00分現在]
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[在庫・価格 ：2025年05月04日 00時00分現在]
Human Serpin A12 Quantikine ELISA Kit
文献数： 0
- 商品コード：DSA120
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：31.3-2,000 pg/mL，感度：14.6 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Saliva, Human Milk，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC156，測定時間：4.5 hours，測定因子：Serpin A12 別名：antitrypsin) Genbank No： 145264 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Human Serpin A12 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL), Human Milk (50 µL)
- Sensitivity
- 14.6 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Serpin A12.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Serpin A12/Vaspin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Serpin A12 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Serpin A12 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Serpin A12 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Serpin A12.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 170 | 531 | 1,061 | 173 | 523 | 1,033 |
| Standard Deviation | 8.57 | 10.6 | 39.9 | 11.2 | 30.6 | 66.6 |
| CV% | 5 | 2 | 3.8 | 6.5 | 5.9 | 6.4 |
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Recovery
The recovery of human Serpin A12 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 98 | 92-107 |
| Serum (n=4) | 92 | 85-99 |
| EDTA Plasma (n=4) | 90 | 80-97 |
| Heparin Plasma (n=4) | 92 | 84-98 |
| Urine (n=4) | 93 | 83-100 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Serpin A12 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Serpin A12
- Aliases
- OL-64; Vaspin
- Entrez Gene IDs
- 145264 (Human); 68054 (Mouse); 191570 (Rat)
- Background
- Serpins
- The human serpin superfamily consists of at least 35 members that target not only serine proteases, but also selected cysteine proteases and non-protease proteins. Serpins bind the protease active site resulting in a major conformational rearrangement that traps the enzyme in a covalent acyl-enzyme intermediate. As protease inhibitors, serpins have an array of functions including regulating blood clotting, the complement pathway, extracellular matrix remodeling, and cell motility. They are also involved in activities that extend beyond their ability to inhibit proteases. For instance, they may also regulate blood pressure, angiogenesis, or act as storage/transport proteins.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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