Human CXCL12/SDF-1 alpha Quantikine ELISA Kit | hCXCL12/SDF-1a Qkit
掲載日情報：2016/08/18 現在Webページ番号：111190
R&D Systems製のHuman CXCL12/SDF-1 alphaを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月01日 12時15分現在]
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SDF-1α, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年05月01日 12時15分現在]
SDF-1α, Human, ELISA Kit, Quantikine (96well)
文献数： 149
- 商品コード：DSA00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：156-10,000 pg/mL，感度：47 pg/mL，測定試料：Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC20，測定時間：4.5 hours，測定因子：CXCL12, SDF-1 alpha 別名：CXCL12 chemokine (C-X-C motif) ligand 12 Genbank No： 6387 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (10 µL), EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
- Sensitivity
- 47 pg/mL
- Assay Range
- 156 - 10,000 pg/mL (Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant SDF-1 alpha
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human SDF-1 alpha Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human SDF-1 alpha in cell culture supernates and platelet-poor plasma. It contains E. coli-expressed recombinant human SDF-1 alpha and antibodies raised against the recombinant factor. It has been shown to accurately quantitate recombinant human SDF-1 alpha. Results obtained using natural SDF-1 alpha showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural SDF-1 alpha.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, EDTA Plasma, Platelet-poor Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 491 | 1,700 | 3,420 | 544 | 1,600 | 3,150 |
| Standard Deviation | 19 | 58 | 115 | 73 | 150 | 257 |
| CV% | 3.9 | 3.4 | 3.4 | 13.4 | 9.4 | 8.2 |
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Recovery
The recovery of SDF-1 alpha spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Platelet-poor EDTA Plasma (n=5) | 97 | 90-107 |
| Platelet-poor Heparin Plasma (n=5) | 97 | 90-104 |
| Cell Culture Media (n=4) | 101 | 95-108 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of SDF-1 alpha were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CXCL12/SDF-1 alpha
- Aliases
- undefined
- Entrez Gene IDs
- 6387 (Human); 20315 (Mouse); 493806 (Feline)
- Background
- CXCL12/SDF-1 (PBSF)
- CXCL12/Stromal Cell-derived Factor 1 (SDF-1) is a member of the CXC chemokine ligand superfamily. Six alternatively spliced CXCL12/SDF-1 isoforms have been identified including, CXCL12/SDF-1 alpha, CXCL12/SDF-1 beta, and CXCL12/SDF-1 gamma. CXCL12/SDF-1 alpha is 89 amino acids (aa) in length with a predicted molecular weight of 10 kDa, CXCL12/SDF-1 beta is 93 aa in length with a predicted molecular weight of 10.6 kDa, and CXCL12/SDF-1 gamma is 119 aa in length with a predicted molecular weight of 13.7 kDa. The mouse CXCL12/SDF-1 orthologs share 99% aa sequence identity with the human protein. The alpha and beta isoforms are ubiquitously expressed while the gamma isoform is primarily expressed in the heart. CXCL12/SDF-1 isoforms are chemotactic for T lymphocytes and monocytes. It is cardio protective and has also been shown to hinder CXCR4-mediated HIV infection of T cells.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Altered features and increased chemosensitivity of human breast cancer cells mediated by adipose tissue-derived mesenchymal stromal cells.
Bohac M, Kozovska Z, Kucerova L, Matuskova M, Skolekova S
BMC Cancer 2013 13:535
Species: Human
Sample Type: Cell Culture Supernates
Intranasal delivery of mesenchymal stem cells significantly extends survival of irradiated mice with experimental brain tumors.
Aboody K, Ahmed A, Balyasnikova I, Ferguson S, Gutova M, Han Y, Lesniak M, Mustafi D, Prasol M, Rincon E, Tobias A, Zhang L
Mol Ther 2013 22:140-8
Species: Human
Sample Type: Cell Lysates
A dynamic inflammatory cytokine network in the human ovarian cancer microenvironment.
Balkwill FR, Bowtell DD, Brennan DJ, Chakravarty P, Charles KA, Coward JI, Gallagher WM, Galletta L, Hagemann T, Kulbe H, Kwong J, Leinster DA, Robinson SC, Salako MA, Schioppa T, Smyth JF, Thompson RG
Cancer Res. 2012 72:66-75
Species: Human
Sample Type: Cell Culture Supernates
Alveolar fibrocyte percentage is an independent predictor of poor outcome in patients with acute lung injury.
Bouadma L, Crestani B, Dehoux M, Elbim C, Garnier M, Gelly J, Lasocki S, Lecon V, Mentre F, Nardelli L, Philip I, Piednoir P, Quesnel C, Soler P
Crit. Care Med. 2012 40:21-8
Species: Human
Sample Type: BALF
Elevated cerebral spinal fluid cytokine levels in boys with cerebral adrenoleukodystrophy correlates with MRI severity.
Lund TC, Miller WP, Orchard PJ, Panoskaltsis-Mortari A, Raymond G, Stadem PS, Tolar J
PLoS ONE 2012 7:e32218
Species: Human
Sample Type: Serum
Breast milk from Tanzanian women has divergent effects on cell-free and cell-associated HIV-1 infection in vitro.
Connor R, Housman M, Howell A, Lee F, Lyimo M, Mosi M, Zain-Ul-Abideen M
PLoS ONE 2012 7:e43815
Species: Human
Sample Type: Milk
Involvement of the CXCR7/CXCR4/CXCL12 axis in the malignant progression of human neuroblastoma.
Coulon A, Flahaut M, Gross N, Joseph J, Liberman J, Muhlethaler-Mottet A, Nyalendo C, Sartelet H, Vassal G
PLoS ONE 2012 7:e43665
Species: Human
Sample Type: Tissue Homogenates
BMP9 regulates endoglin-dependent chemokine responses in endothelial cells.
Brogan , Louise, Conley , Barbara, Liaw , Lucy, Lindner , Volkhard, O'Neill , Christin, Pinz , Ilka, Romero , Diana, Tweedie , Eric, Vary , Calvin P, Young , Kira
Blood 2012 120:4263-73
Species: Human
Sample Type: Cell Culture Supernates
Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Cheng K, Li TS, Malliaras K, Marban E, Marban L, Matsushita N, Sun B, Zhang Y
Cardiovasc. Res. 2011 89:157-65
Species: Human
Sample Type: Cell Culture Supernates
Levels of circulating CD45(dim)CD34(+)VEGFR2(+) progenitor cells correlate with outcome in metastatic renal cell carcinoma patients treated with tyrosine kinase inhibitors.
Billiot F, Escudier B, Farace F, Gross-Goupil M, Hill C, Jacques N, Mauguen A, Taylor M, Tournay E, Vimond N
Br. J. Cancer 2011 104:1144-50
Species: Human
Sample Type: Plasma
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