Human CCL5/RANTES Quantikine ELISA Kit | hRANTES Qkit
掲載日情報：2016/08/18 現在Webページ番号：111185
R&D Systems製のHuman CCL5/RANTESを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月05日 00時00分現在]
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RANTES, Human, ELISA Kit, Quantikine, 2nd Generation (96 well) |
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[在庫・価格 ：2024年05月05日 00時00分現在]
RANTES, Human, ELISA Kit, Quantikine, 2nd Generation (96 well)
文献数： 143
- 商品コード：DRN00B
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：31.20 - 2000 pg/mL，感度：6.6 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：サンドイッチELISA，アッセイ数：96 well，別売品コントロール：#QC20，測定時間：3.5 hours，測定因子：CCL5/RANTES 別名：chemokine (C-C motif) ligand 5 Genbank No： 6352 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL), Citrate Plasma (100 µL), Urine (100 µL)
- Sensitivity
- 6.6 pg/mL
- Assay Range
- 31.2 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human RANTES
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human RANTES Immunoassay is a 3.5 hour solid phase ELISA designed to measure human RANTES levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human RANTES and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RANTES showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human RANTES.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 91.9 | 573 | 1,120 | 92 | 511 | 1,105 |
| Standard Deviation | 2.3 | 10 | 32.4 | 6.2 | 32.7 | 70.7 |
| CV% | 2.5 | 1.7 | 2.9 | 6.7 | 6.4 | 6.4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 108 | 599 | 1,191 | 88 | 494 | 1,063 |
| Standard Deviation | 3.9 | 14.3 | 41.5 | 9.1 | 43.1 | 89.6 |
| CV% | 3.6 | 2.4 | 3.5 | 10.3 | 8.7 | 8.4 |
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Recovery
The recovery of RANTES spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 101 | 95-110 |
| Serum (n=5) | 101 | 94-107 |
| Urine (n=10) | 102 | 88-114 |
| Platelet-poor Citrate Plasma (n=4) | 99 | 93-105 |
| Platelet-poor EDTA Plasma (n=4) | 98 | 93-103 |
| Platelet-poor Heparin Plasma (n=4) | 99 | 93-105 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RANTES were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- CCL5/RANTES
- Aliases
- RANTES; SISd
- Entrez Gene IDs
- 6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
- Background
- CCL5/RANTES
- RANTES, also known as CCL5 and SISd, is a chemokine that belongs to the CC chemokine subfamily. It is a secreted polypeptide produced by a wide variety of epithelial and mesodermal cell types and is involved in both cellular and humoral immunity.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
Conran N, Costa F, Fertrin K, Franco-Penteado C, Olalla Saad S, Pallis F
Br J Haematol 2014 164:286-95
Species: Human
Sample Type: Serum
Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever.
Alves AM, Avila CA, Barbosa LD, da Cunha RV, Damasco PV, de Azeredo EL, de Souza LA, de Souza LJ, de-Oliveira-Pinto LM, Kubelka CF, Marinho CF, Motta-Castro AR, Paes MV, Povoa TF
PLoS ONE 2012 7:e38527
Species: Human
Sample Type: Plasma
Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
J. Thromb. Haemost. 2012 10:1616-23
Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
Aukrust P, Froland SS, Landro L, Otterdal K, Ueland T
J. Thromb. Haemost. 2011 9:1075-7
Species: Human
Sample Type: Platelet-Poor Plasma
A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets.
Amiri KI, Horton LW, Su Y
Clin. Cancer Res. 2010 16:348-57
Species: Human
Sample Type: Serum
Human CD4- 8- T cells are a distinctive immunoregulatory subset.
Goetzl EJ, Huang MC, Longo DL, Patel K, Taub DD
FASEB J. 2010 24:2558-66
Species: Human
Sample Type: Cell Culture Supernates
Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
Law HK, Tso GH, Tu W
Stem Cells 2010 28:939-54
Species: Human
Sample Type: Cell Culture Supernates
Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
Kweon SH, Kwon KB, Lee YR, Park BH, Park JW, Yoon TR
Biochem. Biophys. Res. Commun. 2009 378:90-4
Species: Human
Sample Type: Cell Culture Supernates
Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
Graham CH, Renaud SJ, Sullivan R
Placenta 2009 30:313-9
Species: Human
Sample Type: Cell Culture Supernates
HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
Brandt O, Elbe-Burger A, Eppel W, Fiala C, Imhof M, Meindl S, Schuster C, Stingl G, Vaculik C
J. Exp. Med. 2009 206:169-81
Species: Human
Sample Type: Cell Culture Supernates
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