Human RAGE Quantikine ELISA Kit | hRAGE Qkit
掲載日情報：2016/08/18 現在Webページ番号：111183
R&D Systems製のHuman RAGEを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月28日 16時55分現在]
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RAGE, Human, ELISA Kit, Quantikine (96 well) |
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[在庫・価格 ：2025年04月28日 16時55分現在]
RAGE, Human, ELISA Kit, Quantikine (96 well)
文献数： 71
- 商品コード：DRG00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：78-5,000 pg/mL，感度：16.14 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC152，測定時間：4.5 hours，測定因子：RAGE, AGER 別名：AGER Genbank No： 5891 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2022年11月1日号 p.12
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| 製品記事 | Quantikine Human RAGE ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 16.14 pg/mL
- Assay Range
- 78 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human RAGE
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human RAGE Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human RAGE (extracellular domain) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human RAGE/Fc Chimera and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RAGE showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring RAGE.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 571 | 1,549 | 3,189 | 563 | 1,568 | 3,159 |
| Standard Deviation | 34 | 73 | 191 | 49 | 110 | 193 |
| CV% | 5.9 | 4.7 | 6 | 8.7 | 7 | 6.1 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 546 | 1,527 | 3,117 | 519 | 1,449 | 2,890 |
| Standard Deviation | 34 | 73 | 189 | 43 | 119 | 192 |
| CV% | 6.2 | 4.8 | 6.1 | 8.2 | 8.2 | 6.7 |
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Recovery
The recovery of RAGE spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Tissue Culture Media (n=4) | 98 | 86-108 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RAGE were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- RAGE
- Aliases
- AGER
- Entrez Gene IDs
- 177 (Human); 11596 (Mouse); 81722 (Rat)
- Background
- RAGE
- RAGE (receptor for advanced glycation end products) is a multi-ligand type I transmembrane glycoprotein belonging to the immunoglobulin (Ig) superfamily. RAGE ligands include advanced glycation end products (AGEs), amyloid-beta (A beta) peptide, HMG-1 (also known as Amphoterin), and several members of the S100 protein superfamily. RAGE has potential involvement in several pathological processes including diabetes, Alzheimer's disease (AD), systemic amyloidosis, and tumor growth. RAGE may also mediate several physiological functions such as neuronal outgrowth, survival, and regeneration, and play a part in pro-inflammatory reactions.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Targeted gene silencing using a follicle-stimulating hormone peptide-conjugated nanoparticle system improves its specificity and efficacy in ovarian clear cell carcinoma in vitro.
Chen J, Hong S, Xu C, Zhang X, Zheng Y, Zhou J
J Ovarian Res 2013 6:80
Species: Human
Sample Type: Cell Culture Supernates
Soluble RAGE as a severity marker in community acquired pneumonia associated sepsis.
Narvaez-Rivera RM, Rendon A, Salinas-Carmona MC
BMC Infect. Dis. 2012 12:15
Species: Human
Sample Type: Serum
Methodological and Preanalytical Evaluation of a RAGE Immunoassay.
Fersching D, Holdenrieder S, Lehner J, Siegele B, Stoetzer OJ, Wittwer C
Anticancer Res. 2012 32:2075-8
Species: Human
Sample Type: Serum
Diastolic dysfunction of aging is independent of myocardial structure but associated with plasma advanced glycation end-product levels.
Black M, Campbell D, Jenkins A, Kelly D, Kenny J, Newcomb A, Prior D, Schalkwijk C, Somaratne J, Yii M
PLoS ONE 2012 7:e49813
Species: Human
Sample Type: Plasma
The soluble form of the receptor of advanced glycation endproducts increases after bariatric surgery in morbid obesity.
Brix J, Hollerl F, Kopp H, Schernthaner G
Int J Obes (Lond) 2012 36:1412-7
Species: Human
Sample Type: Serum
Soluble form of the receptor for advanced glycation end products is a marker of acute lung injury but not of severe sepsis in critically ill patients.
Futier E, Jabaudon M, Roszyk L
Crit. Care Med. 2011 39:480-8
Species: Human
Sample Type: Plasma
Total soluble and endogenous secretory receptor for advanced glycation end products as predictive biomarkers of coronary heart disease risk in patients with type 2 diabetes: an analysis from the CARDS trial.
Bao W, Betteridge DJ, Charlton-Menys V, Colhoun HM, Demicco DA, Deshmukh H, Durrington P, Fuller JH, Hitman G, Livingstone S, Neil A, Preston GM, Tan K
Diabetes 2011 60:2379-85
Species: Human
Sample Type: Serum
Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
Makita K, Matthay MA, Uchida T, Yamakawa N
Am. J. Physiol. Lung Cell Mol. Physiol. 2011 300:L516-25
Species: Human
Sample Type: pulmonary edema fluid
Effects of atorvastatin on serum soluble receptors for advanced glycation end-products in type 2 diabetes.
Betteridge DJ, Chow WS, Shiu SW, Tam HL, Tan KC, Wong Y
Atherosclerosis 2010 209:173-7
Species: Human
Sample Type: Serum
Circulating high-molecular-weight RAGE ligands activate pathways implicated in the development of diabetic nephropathy.
Coughlan MT, Patel SK, Penfold SA
Kidney Int. 2010 78:287-95
Species: Human
Sample Type: Serum
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