Human RBP4 Quantikine ELISA Kit | hRBP4 QKit
掲載日情報：2016/08/18 現在Webページ番号：111181
R&D Systems製のHuman RBP4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時55分現在]
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RBP4, Human, ELISA Kit, Quantikine (96 well) <Retinol-Binding Protein 4, ELISA Kit> |
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[在庫・価格 ：2025年04月29日 20時55分現在]
RBP4, Human, ELISA Kit, Quantikine (96 well) <Retinol-Binding Protein 4, ELISA Kit>
文献数： 18
- 商品コード：DRB400
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：1.56-100 ng/mL，感度：0.628 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Saliva，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC23，測定時間：2.5 hours，測定因子：RBP4, Retinol-Binding Protein 4 別名：interstitial Genbank No： 5950 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Retinol-binding Protein 4 / RBP4 Quantikine® ELISA kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 2.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (20 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (10 µL)
- Sensitivity
- 0.628 ng/mL
- Assay Range
- 1.56 - 100 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human RBP4
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human RBP4 immunoassay is a 2.5 hour solid phase ELISA designed to measure human RBP4 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human RBP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RBP4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human RBP4.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 11 | 30.3 | 61.9 | 9.8 | 27.5 | 54.7 |
| Standard Deviation | 0.77 | 1.73 | 5.01 | 0.84 | 1.6 | 4 |
| CV% | 7 | 5.7 | 8.1 | 8.6 | 5.8 | 7.3 |
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Recovery
The recovery of RBP4 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 102 | 86-115 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RBP4 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- RBP4/Retinol-Binding Protein 4
- Aliases
- undefined
- Entrez Gene IDs
- 5950 (Human); 19662 (Mouse); 25703 (Rat)
- Background
- Retinol-binding Protein 4/RBP4
- Retinol (also known as vitamin A) is unstable and insoluble in aqueous solution. However, it becomes stable and soluble in plasma due to its tight interaction with RBP4, also known as plasma retinol-binding protein. A prototypic member of the lipocalin superfamily, RBP4 has a beta-barrel structure with a well-defined cavity. It is secreted from the liver in a process requiring the availability of retinol. RBP4 delivers retinol from the liver to the peripheral tissues. In plasma, RBP4-retinol forms a complex with transthyretin (TTR), also known as thyroxine-binding protein and prealbumin. Defects in RBP4 cause retinol-binding protein deficiency, which affects night vision.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Adipocytokine levels mark endothelial function in normotensive individuals.
Bruno R, Duranti E, Ghiadoni L, Santini E, Solini A, Stea F, Taddei S
Cardiovasc Diabetol 2012 11:103
Species: Human
Sample Type: Plasma
Increased retinol-binding protein (RBP) 4 and anti-RBP4 antibody in alopecia areata.
Ahn KJ, Bang D, Cho SB, Choi EA, Kim J, Lee JH, Lee KH
Br. J. Dermatol. 2011 165:837-44
Species: Human
Sample Type: Serum
Kinetics of circulating Th17 cytokines and adipokines in psoriasis patients.
Morishige R, Nakajima H, Nakajima K, Sano S, Tarutani M
Arch. Dermatol. Res. 2011 303:451-5
Species: Human
Sample Type: Serum
Nonalcoholic fatty liver disease as the transducer of hepatic oversecretion of very-low-density lipoprotein-apolipoprotein B-100 in obesity.
Chan DC, Gan S, Watts GF
Arterioscler. Thromb. Vasc. Biol. 2010 30:1043-50
Species: Human
Sample Type: Plasma
Retinol-binding protein-4 in women with untreated essential hypertension.
Madec S, Muscelli E, Rossi C, Santini E, Solini A
Am. J. Hypertens. 2009 22:1001-6
Species: Human
Sample Type: Serum
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