Human Pappalysin-1/PAPP-A Quantikine ELISA Kit | hPappalysin-1 QKit
掲載日情報：2016/08/18 現在Webページ番号：111173
R&D Systems製のHuman Pappalysin-1/PAPP-Aを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時55分現在]
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Pappalysin-1/PAPP-A, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月29日 20時55分現在]
Pappalysin-1/PAPP-A, Human, ELISA Kit, Quantikine (96well)
文献数： 4
- 商品コード：DPPA00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.78-50 ng/mL，感度：0.116 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC23，測定時間：5.5 hours，測定因子：Pappalysin-1, PAPP-A 別名：ASBABP2 Genbank No： 5069 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Pappalysin-1/PAPP-A Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 5.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 0.116 ng/mL
- Assay Range
- 0.78 - 50 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human PAPP-A
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human PAPP-A Immunoassay is a 5.5 hour sandwich-type solid phase ELISA designed to measure human PAPP-A in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human PAPP-A and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PAPP-A showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PAPP-A.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 5.49 | 17.4 | 32.7 | 5.2 | 16.4 | 31.4 |
| Standard Deviation | 0.32 | 0.78 | 1.39 | 0.44 | 1.14 | 2.04 |
| CV% | 5.8 | 4.5 | 4.3 | 8.5 | 6.9 | 6.5 |
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Recovery
The recovery of PAPP-A spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 92 | 84-104 |
| Heparin Plasma (n=4) | 95 | 86-111 |
| EDTA Plasma (n=4) | 97 | 89-108 |
| Cell Culture Media (n=4) | 110 | 90-117 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PAPP-A were serially diluted in Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Pappalysin-1/PAPP-A
- Aliases
- ASBABP2; DIPLA1; IGFBP-4ase; PAPA; PAPPA; PAPP-A1
- Entrez Gene IDs
- 5069 (Human)
- Background
- Pappalysins
- Pappalysins comprise one of the five families of Metzincins (other families include ADAMs/ADAMTSs, MMPs, Astacins, and Serralysins). Pappalysin-1 (PAPP-A) is an important pregnancy protein and increases in plasma by a factor of about 150 during pregnancy. Reduced maternal serum PAPP-A is a major marker of Down syndrome in the first trimester of pregnancy. PAPP-A cleaves IGFBP-4 and -5 (insulin-like growth factor-binding protein-4 and 5) at a single site, resulting in the release of bioactive IGF. Pappalysin-2 (PAPP-A2; also called PAPP-E) shares 45% amino acid identity with PAPP-A and cleaves IGFBP-2.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
A proof-of-principle gel-free proteomics strategy for the identification of predictive biomarkers for the onset of pre-eclampsia.
Blankley RT, Gaskell SJ, Whetton AD
BJOG 2009 116:1473-80
Species: Human
Sample Type: Plasma
Increased levels of pregnancy-associated plasma protein-A2 in the serum of pre-eclamptic patients.
Nishizawa H, Pryor-Koishi K, Suzuki M
Mol. Hum. Reprod. 2008 14:595-602
Species: Human
Sample Type: Serum
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