Human Progranulin Quantikine ELISA Kit | hProgranulin QKit
掲載日情報：2016/08/18 現在Webページ番号：111169
R&D Systems製のHuman Progranulinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月28日 16時55分現在]
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Progranulin, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月28日 16時55分現在]
Progranulin, Human, ELISA Kit, Quantikine (96well)
文献数： 14
- 商品コード：DPGRN0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：1.56-100 ng/mL，感度：0.54 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Saliva, Human Milk，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC147，測定時間：4.5 hours，測定因子：Progranulin, PGRN 別名：Acrogranin, GEP, GP88, GRN, PCDGF, PEPI, PGRN, Proepithelin Genbank No： 2896 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Progranulin Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (25 µL), Human Milk (10 µL)
- Sensitivity
- 0.54 ng/mL
- Assay Range
- 1.56 - 100 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Progranulin. Trypsin and Elastase cleaved Progranulin show reduced recognition in this assay, demonstrating specificity for Progranulin.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Progranulin immunoassay is a 4.5 hour solid phase ELISA designed to measure Progranulin in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human Progranulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Progranulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Progranulin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 15.5 | 36.6 | 70.8 | 15.7 | 35.8 | 69.1 |
| Standard Deviation | 0.9 | 1.7 | 3.1 | 1.3 | 2.9 | 5.1 |
| CV% | 5.8 | 4.6 | 4.4 | 8.3 | 8.1 | 7.4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 13.5 | 32.7 | 64.8 | 14.4 | 33.1 | 64.4 |
| Standard Deviation | 0.7 | 1.9 | 4.1 | 1 | 2.3 | 4.4 |
| CV% | 5.2 | 5.8 | 6.3 | 6.9 | 6.9 | 6.8 |
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Recovery
The recovery of Progranulin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 104 | 90-115 |
| Heparin Plasma (n=4) | 100 | 92-108 |
| EDTA Plasma (n=4) | 102 | 95-110 |
| Urine (n=4) | 109 | 99-116 |
| Cell Culture Media (n=4) | 107 | 103-112 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Progranulin were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Progranulin/PGRN
- Aliases
- Acrogranin; GEP; GP88; GRN; PCDGF; PEPI; PGRN; Proepithelin
- Entrez Gene IDs
- 2896 (Human); 14824 (Mouse)
- Background
- Progranulin
- Progranulin, also known as proepithelin and PC cell-derived growth factor (PCDGF), is a secreted, glycosylated protein with multiple repeats of the granulin/epithelin motif.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Regulation of progranulin expression in human microglia and proteolysis of progranulin by matrix metalloproteinase-12 (MMP-12).
Choi N, Lee SC, Suh HS, Tarassishin L
PLoS ONE 2012 7:e35115
Species: Human
Sample Type: Cell Culture Supernates
p38 MAPK alpha mediates cytokine-induced IL-6 and MMP-3 expression in human cardiac fibroblasts.
Biochem Biophys Res Commun 2012 430:419-24
Species: Mouse
Sample Type: cell supernates
Pentraxin-3 Is a Novel Biomarker of Lung Carcinoma.
Diamandis EP, Goodglick L, Planque C, Thornquist MD
Clin. Cancer Res. 2011 17:2395-2399
Species: Human
Sample Type: Serum
Low plasma progranulin levels in children with autism.
, Al-Ayadhi LY, Mostafa GA
J Neuroinflammation 2011 8:111
Species: Human
Sample Type: Plasma
Genome-wide screen identifies rs646776 near sortilin as a regulator of progranulin levels in human plasma.
Baker M, Bisceglio GD, Carrasquillo MM, Cookson MR, Crook JE, DeJesus-Hernandez M, Dillman A, Finch N, Gibbs JR, Graff-Radford NR, Hernandez D, Hunter TA, Mackenzie IR, Nicholson AM, Petersen RC, Rademakers R, Rutherford NJ, Singleton A, Younkin SG
Am. J. Hum. Genet. 2010 87:890-7
Species: Human
Sample Type: Plasma
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