Human Proprotein Convertase 9/PCSK9 Quantikine ELISA Kit | hPC9 QKit
掲載日情報：2016/08/18 現在Webページ番号：111166
R&D Systems製のHuman Proprotein Convertase 9/PCSK9を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時35分現在]
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Proprotein Convertase 9, Human, ELISA Kit, Quantikine (96well) <PCSK9, ELISA Kit> |
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[在庫・価格 ：2025年04月29日 20時35分現在]
Proprotein Convertase 9, Human, ELISA Kit, Quantikine (96well) <PCSK9, ELISA Kit>
文献数： 28
- 商品コード：DPC900
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.625-40 ng/mL，感度：0.219 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Lysate，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC235，測定時間：4.5 hours，測定因子：Fibroblast Activation Protein alpha, FAP 別名：EC 3.4.21 Genbank No： 255738 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Human Proprotein Convertase 9/PCSK9 Quantikine® ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 0.219 ng/mL
- Assay Range
- 0.625 - 40 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Natural and recombinant human PCSK9. This assay recognizes free and LDLR-bound PCSK9.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human PCSK9 immunoassay is a 4.5 hour solid phase ELISA designed to measure PCSK9 in cell culture supernates, cell lysates, serum, and plasma. It contains NS0-expressed recombinant human PCSK9 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural PCSK9 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring PCSK9.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 4.82 | 14 | 27.7 | 4.64 | 14.5 | 27.9 |
| Standard Deviation | 0.196 | 0.795 | 1.81 | 0.276 | 0.629 | 1.13 |
| CV% | 4.1 | 5.7 | 6.5 | 6 | 4.3 | 4.1 |
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Recovery
The recovery of PCSK9 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 107 | 100-111 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of PCSK9 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Proprotein Convertase 9/PCSK9
- Aliases
- FH3; HCHOLA3; NARC-1; PC9; PCSK9
- Entrez Gene IDs
- 255738 (Human); 100102 (Mouse); 298296 (Rat)
- Background
- Proprotein Convertases
- This family of serine proteases consists of furin and proprotein convertases encoded by PCSK genes. They are important in a variety of processes. Furin has an essential role in embryogenesis and homeostasis and is implicated in various pathologies such as cancer, neurodegenerative diseases and anthrax. Mutations in the PC1/PCSK1 gene are associated with obesity and impaired prohormone processing.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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