Human Neuropilin-1 Quantikine ELISA Kit | hNeuropilin-1 QKit
掲載日情報：2016/08/18 現在Webページ番号：111160
R&D Systems製のHuman Neuropilin-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年06月19日 08時15分現在]
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[在庫・価格 ：2024年06月19日 08時15分現在]
Human Neuropilin-1 Quantikine ELISA Kit
文献数： 1
- 商品コード：DNRP10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
別売品コントロール：QC187，感度：9.33 pg/mL，測定範囲：78.10 - 5000 pg/mL，測定試料：Cell Culture Supernates (50 uL), Cell Lysates (13 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Urine (50 uL), Human Milk (10 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，測定時間：4.5 hours，測定因子：Neuropilin-1 別名：BDCA4 Genbank No： 8829 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Human Neuropilin-1 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (13 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL), Human Milk (10 µL)
- Sensitivity
- 9.33 pg/mL
- Assay Range
- 78.1 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Human Milk, Cell Lysates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human Neuropilin-1.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Neuropilin-1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Neuropilin-1 in cell culture supernates, cell lysates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human Neuropilin-1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Neuropilin-1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Neuropilin-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 336 | 967 | 1,890 | 329 | 934 | 1,833 |
| Standard Deviation | 17.9 | 70.4 | 54.7 | 19.8 | 48.4 | 90.4 |
| CV% | 5.3 | 7.3 | 2.9 | 6 | 5.2 | 4.9 |
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Recovery
The recovery of human Neuropilin-1 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 91 | 86-99 |
| Cell Lysates (n=3) | 101 | 94-108 |
| Urine (n=4) | 99 | 85-117 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Neuropilin-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Neuropilin-1
- Aliases
- BDCA-4; CD304; NRP1
- Entrez Gene IDs
- 8829 (Human); 18186 (Mouse); 246331 (Rat)
- Background
- Neuropilins
- Neuropilin-1 (Npn-1, previously known as neuropilin) and Neuropilin-2 (previously known as Npn-1-related molecule) are type I transmembrane proteins that bind distinct members of the class III secreted semaphorin subfamily that are implicated in repulsive axon guidance. Neuropilin extracellular domains contain two CUB (complement-binding) domains, two domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain. In the absence of ligands, neuropilins can form homo- and hetero-oligomers via their MAM domains. Expression in developing neurons of the central and peripheral nervous systems is somewhat overlapping but distinct. Npn-1 and Npn-2 are also receptors for VEGF165 on endothelial and tumor cells.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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