Human Mesothelin Quantikine ELISA Kit | hMesothelin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111156
R&D Systems製のHuman Mesothelinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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Mesothelin, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
Mesothelin, Human, ELISA Kit, Quantikine (96well)
文献数： 2
- 商品コード：DMSLN0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 |
測定範囲：0.16 - 10 ng/mL，感度：0.022 ng/mL，測定試料：Cell Culture Supernates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (50 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：Mesothelin 別名：CAK1 Genbank No： 10232 |
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| 法規制等 | 医薬用外毒物 | ||
| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human Mesothelin ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (50 µL)
- Sensitivity
- 0.022 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine, Saliva)
- Specificity
- Natural and recombinant human Mesothelin
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Mesothelin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Mesothelin in cell culture supernates, serum, plasma, saliva, and urine. It contains NS0-expressed recombinant human Mesothelin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Mesothelin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Mesothelin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 0.83 | 2.56 | 5.54 | 0.91 | 2.8 | 5.94 |
| Standard Deviation | 0.034 | 0.082 | 0.193 | 0.057 | 0.13 | 0.36 |
| CV% | 4 | 3.2 | 3.5 | 6.2 | 4.6 | 6.1 |
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Recovery
The recovery of Mesothelin spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 99 | 92-114 |
| Heparin Plasma (n=4) | 97 | 86-112 |
| Saliva (n=4) | 102 | 91-112 |
| EDTA Plasma (n=4) | 97 | 88-109 |
| Urine (n=4) | 103 | 86-115 |
| Cell Culture Media (n=8) | 104 | 91-113 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of Mesothelin were serially diluted Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Mesothelin
- Aliases
- CAK1; MPF; MSLN; SMR
- Entrez Gene IDs
- 10232 (Human); 56047 (Mouse); 60333 (Rat)
- Background
- Mesothelin/MPF
- Mesothelin is a glycosylphosphatidylinositol-anchored glycoprotein that is expressed on mesothelial cells in the pleura, pericardium and peritoneum and overexpressed in mesotheliomas and ovarian or pancreatic adenocarcinomas. Mesothelin is a product of the CAK-1 gene, which also encodes megakaryocyte-potentiating factor (MPF). Mature human mesothelin shares 60% amino acid identity with either mouse or rat mesothelin. Two variant forms exist; variant 1 has an eight amino acid (1 kDa) insertion and is rarely expressed, while variant 2 is a truncated form secreted in the majority of ovarian cancers but rarely found in normal individuals.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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