Human MMP-9 Quantikine ELISA Kit | hMMP-9 QKit
掲載日情報：2016/08/18 現在Webページ番号：111155
R&D Systems製のHuman MMP-9を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月18日 00時00分現在]
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MMP-9, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月18日 00時00分現在]
MMP-9, Human, ELISA Kit, Quantikine (96well)
文献数： 252
- 商品コード：DMP900
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：1個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：0.31 - 20 ng/mL，感度：0.156 ng/mL，測定試料：Cell Culture Supernates (10 uL), Serum (10 uL), Platelet-poor Heparin Plasma (10 uL), Saliva (10 uL), Urine (100 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC130，測定時間：3.5 hours，測定因子：MMP-9 別名：92 kDa gelatinase Genbank No： 4318 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (10 µL), Serum (10 µL), Platelet-poor Heparin Plasma (10 µL), Saliva (10 µL), Urine (100 µL)
- Sensitivity
- 0.156 ng/mL
- Assay Range
- 0.312 - 20 ng/mL (Serum, Saliva, Cell Culture Supernates, Platelet-poor Heparin Plasma, Urine)
- Specificity
- Natural and recombinant human 92 kDa Pro-MMP-9 and the 82 kDa active MMP-9
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human MMP-9 Immunoassay is a 3.5 hour solid phase ELISA designed to measure MMP-9 (92 kDa pro- and 82 kDa active forms but not the 65 kDa form) in cell culture supernates, saliva, serum, plasma, and urine. It is calibrated with CHO cell-expressed recombinant human pro-MMP-9 and the antibodies were raised against the recombinant factor. Natural human MMP-9 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human MMP-9.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Platelet-poor Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.833 | 2.04 | 11 | 0.972 | 2.35 | 12.2 |
| Standard Deviation | 0.017 | 0.039 | 0.316 | 0.077 | 0.184 | 0.845 |
| CV% | 2 | 1.9 | 2.9 | 7.9 | 7.8 | 6.9 |
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Recovery
The recovery of Pro-MMP-9 spiked to three levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 95 | 91-99 |
| Saliva (n=4) | 90 | 74-114 |
| Platelet-poor Heparin Plasma (n=5) | 96 | 89-108 |
| Urine (n=5) | 91 | 82-99 |
| Cell Culture Media (n=5) | 97 | 85-104 |
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Linearity
To assess the linearity of the assay, samples spiked with or containing high concentrations of Pro-MMP-9 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- MMP-9
- Aliases
- CLG4B; Gelatinase B; GELB
- Entrez Gene IDs
- 4318 (Human); 17395 (Mouse); 81687 (Rat)
- Background
- Matrix Metalloproteinases (MMPs)
- The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Validation and quality control of ELISAs for the use with human saliva samples.
Jaedicke KM, Preshaw PM, Taylor JJ
J. Immunol. Methods 2012 377:62-5
Cerebrospinal Fluid Neopterin as Marker of the Meningo-Encephalitic Stage of Trypanosoma brucei gambiense Sleeping Sickness.
Bisser S, Buscher P, Courtioux B, Enyaru JC, Hainard A, Krishna S, Kristensson K, Lejon V, Matovu E, Ndung'u JM, Ngoyi DM, Robin X, Sanchez JC, Tiberti N, Turck N, Vatunga GM
PLoS ONE 2012 7:e40909
Species: Human
Sample Type: CSF
A multi-analyte assay for the non-invasive detection of bladder cancer.
Chang , Myron, Dai , Yunfeng, Goodison , Steve, Rosser , Charles , Urquidi , Virginia
PLoS ONE 2012 7:e47469
Species: Human
Sample Type: Urine
Biomarkers of extracellular matrix metabolism (MMP-9 and TIMP-1) and risk of stroke, myocardial infarction, and cause-specific mortality: cohort study.
Arnlov J, Hansson J, Ingelsson E, Larsson A, Lind L, Michaelsson K, Sundstrom J, Vasan RS
PLoS ONE 2011 6:e16185
Species: Human
Sample Type: Serum
Characterization of innate immune signalling receptors in virus-induced acute asthma.
Gibson PG, Powell H, Simpson JL, Wark PA, Wood LG
Clin. Exp. Allergy 2011 41:640-8
Species: Human
Sample Type: Saliva
Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
Kumar M, Nerurkar VR, Verma S
J. Gen. Virol. 2011 92:507-15
Species: Human
Sample Type: Cell Culture Supernates
Increased levels of leukocyte-derived MMP-9 in patients with stable angina pectoris.
Bergstrom I, Jonasson L, Jonasson S, Kalvegren H, Lundberg A, Szymanowski A
PLoS ONE 2011 6:e19340
Species: Human
Sample Type: Serum
Leptin administered in physiological or pharmacological doses does not regulate circulating angiogenesis factors in humans.
Aronis KN, Diakopoulos KN, Fiorenza CG
Diabetologia 2011 54:2358-67
Species: Human
Sample Type: Serum
Double-blind, randomized, multicentre, and active comparator controlled investigation of the effect of Pioglitazone, Metformin, and the combination of both on cardiovascular risk in patients with type 2 diabetes receiving stable basal insulin therapy: the PIOCOMB study.
Forst T, Fuchs W, Hanefeld M, Kleine I, Pfutzner A
Cardiovasc Diabetol 2011 10:65
Species: Human
Sample Type: Serum
Abnormalities in metalloproteinase pathways and IGF-I axis: a link between birth weight, hypertension, and vascular damage in childhood.
Franco MC, Sesso R
Am. J. Hypertens. 2010 23:6-11
Species: Human
Sample Type: Plasma
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