Human Pro-MMP-1 Quantikine ELISA Kit | hPro-MMP-1 QKit
掲載日情報：2016/08/18 現在Webページ番号：111151
R&D Systems製のHuman Pro-MMP-1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年05月14日 14時15分現在]
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MMP-1, Pro-, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年05月14日 14時15分現在]
MMP-1, Pro-, Human, ELISA Kit, Quantikine (96well)
文献数： 65
- 商品コード：DMP100
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.156-10 ng/mL，感度：0.095 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC125，測定時間：4.5 hours，測定因子：Pro MMP-1 別名：CLGmatrix metalloprotease 1 Genbank No： 4312 |
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| 保存条件 | 4℃ | 法規備考 | |||||
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| 製品記事 | ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 0.095 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates)
- Specificity
- Natural and recombinant human Pro-MMP-1
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Pro-MMP-1 Immunoassay is a 4.5 hour solid phase ELISA designed to measure Pro-MMP-1 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-1 and antibodies raised against the recombinant factor. The antibodies will not recognize recombinant human active MMP-1 or TIMP bound MMP-1. Natural human Pro-MMP-1 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative mass values of natural human Pro-MMP-1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.494 | 1.65 | 3.39 | 0.561 | 1.73 | 3.5 |
| Standard Deviation | 0.022 | 0.065 | 0.177 | 0.065 | 0.153 | 0.27 |
| CV% | 4.5 | 4 | 5.2 | 11.7 | 8.8 | 7.7 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 0.702 | 2.13 | 4.43 | 0.738 | 2.34 | 4.66 |
| Standard Deviation | 0.039 | 0.13 | 0.241 | 0.077 | 0.176 | 0.312 |
| CV% | 5.6 | 6.1 | 5.4 | 10.4 | 7.5 | 6.7 |
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Recovery
The recovery of Pro-MMP-1 spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 97 | 87-112 |
| Heparin Plasma (n=5) | 92 | 85-102 |
| Cell Culture Media (n=4) | 103 | 98-112 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Pro-MMP-1 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.\line 
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Additional Infomation
- Molecule Information
- MMP-1
- Aliases
- undefined
- Entrez Gene IDs
- 4312 (Human)
- Background
- Matrix Metalloproteinases (MMPs)
- The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Anti-arthritic effects of magnolol in human interleukin 1??-stimulated fibroblast-like synoviocytes and in a rat arthritis model.
Chang AS, Liou JP, Shih KS, Tsai CL, Wang JH, Wang KL, Wu YW, Yang CR
PLoS ONE 2012 7:e31368
Species: Human
Sample Type: Cell Culture Supernates
Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade.
Androudi S, Brazitikos P, Dimitrakos SA, Diza E, Karakiulakis G, Papakonstantinou E, Symeonidis C, Tsaousis KT, Tsinopoulos I
Cytokine 2012 59:184-90
Species: Human
Sample Type: Subretinal fluid
Myoferlin depletion in breast cancer cells promotes mesenchymal to epithelial shape change and stalls invasion.
Ackerman WE, Li R, Mihai C
PLoS ONE 2012 7:e39766
Species: Human
Sample Type: Cell Culture Supernates
Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
Bruserud O, Hatfield KJ, Kalland KH, Kittang AO, Oyan AM, Reikvam H
Eur. J. Haematol. 2010 84:239-51
Species: Human
Sample Type: Cell Culture Supernates
High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
Hashizume M, Mihara M
Biochem. Biophys. Res. Commun. 2010 403:184-9
Species: Human
Sample Type: Cell Culture Supernates
Proteinase-activated receptor (PAR)-2 activation impacts bone resorptive properties of human osteoarthritic subchondral bone osteoblasts.
Amiable N, Boileau C, Duval N, Lajeunesse D, Martel-Pelletier J, Pelletier JP, Tat SK
Bone 2009 44:1143-50
Species: Human
Sample Type: Cell Culture Supernates
Serum matrix metalloproteinases in adult CF patients: Relation to pulmonary exacerbation.
Graf J, Rath T, Roderfeld M, Roeb E, Schulz R, Seeger W, Tschuschner A
J. Cyst. Fibros. 2009 8:338-47
Species: Human
Sample Type: Serum
Establishment of a matrix-associated transepithelial resistance invasion assay to precisely measure the invasive potential of synovial fibroblasts.
Dankbar B, Freyth K, Hansen U, Neugebauer K, Pap T, Pundt N, Schnaeker EM, Wendholt D, Wunrau C
Arthritis Rheum. 2009 60:2606-11
Species: Human
Sample Type: Cell Culture Supernates
PI3Kgamma regulates cartilage damage in chronic inflammatory arthritis.
Gay S, Hayer S, Joosten LA, Korb A, Kuhnel I, Neugebauer K, Pap T, Penninger J, Peters MA, Pundt N, Ruckle T, Schett G, Strietholt S, Wunrau C, Zwerina J
FASEB J. 2009 23:4288-98
Species: Human
Sample Type: Cell Culture Supernates
Serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia.
Anastasiadis K, Antonitsis P, Argiriadou H, Charokopos N, Foroulis CN, Karapanagiotidis GT, Papakonstantinou C, Rammos K, Rouska E
J Cardiothorac Surg 2009 4:59
Species: Human
Sample Type: Serum
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