Human MIA Quantikine ELISA Kit | hMIA QKit

掲載日情報:2016/08/18 現在Webページ番号:111150

R&D Systems製のHuman MIAを測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2024年03月29日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。
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納期 文献数
MIA, Human, ELISA Kit, Quantikine (96 well)
10日程度 ※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。 1
説明文
種交差性:Human,感度:8.46 pg/mL,測定範囲:15.60 - 1000 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (50 uL),測定波長:450 nm,検出方法:呈色,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC121,測定時間:4.5 hours,測定因子:MIA
別名:CD-RAP
Genbank No: 8190
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Quantikine Human MIA ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

[在庫・価格 :2024年03月29日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

MIA, Human, ELISA Kit, Quantikine (96 well)

文献数: 1

説明文 種交差性:Human,感度:8.46 pg/mL,測定範囲:15.60 - 1000 pg/mL,測定試料:Cell Culture Supernates (50 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Saliva (50 uL),測定波長:450 nm,検出方法:呈色,アッセイ法:Solid Phase Sandwich ELISA,アッセイ数:96 well,別売品コントロール:#QC121,測定時間:4.5 hours,測定因子:MIA
別名:CD-RAP
Genbank No: 8190
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Quantikine Human MIA ELISA Kit
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL), Saliva (50 µL)

Sensitivity
8.46 pg/mL
Assay Range
15.6 - 1,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, EDTA Plasma)
Specificity
Natural and recombinant human MIA
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Human MIA Immunoassay is a 4.5 hour solid-phase ELISA designed to measure MIA in cell culture supernates, serum, plasma, and saliva. It contains E. coli-expressed recombinant Human MIA and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MIA. Results obtained using natural human MIA showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MIA.

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Precision

Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 158 315 622 150 308 627
Standard Deviation 5.5 10.9 17.8 7.02 15.7 37.8
CV% 3.5 3.5 2.9 4.7 5.1 6

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Recovery

The recovery of MIA spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 103 101-107
Serum (n=4) 101 91-118
EDTA Plasma (n=4) 98 85-115
Heparin Plasma (n=4) 99 85-113
Saliva (n=4) 102 86-110

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Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of MIA were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

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Additional Infomation

Molecule Information
MIA
Aliases
CD-RAP
Entrez Gene IDs
8190 (Human); 12587 (Mouse)
Background
Melanoma Inhibitory Activity (MIA)
MIA, also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is a secreted protein expressed in cartilaginous tissue during development and in the adult. MIA was identified based on the fact that it is highly expressed in malignant melanomas, but not in normal melanocytes. MIA has been shown to interact with fibronectin, and promotes invasion and metastasis of melanoma cells, perhaps by blocking integrin-fibronectin interactions.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

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