Human Leptin Quantikine ELISA Kit | hLeptin QKit
掲載日情報：2016/08/18 現在Webページ番号：111139
R&D Systems製のHuman Leptinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月24日 15時15分現在]
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Leptin, Human, ELISA Kit, Quantikine (96 well) <OB, ELISA Kit> |
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[在庫・価格 ：2024年04月24日 15時15分現在]
Leptin, Human, ELISA Kit, Quantikine (96 well) <OB, ELISA Kit>
文献数： 142
- 商品コード：DLP00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：2個
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：15.60 - 1000 pg/mL，感度：7.8 pg/mL，測定試料：Cell Culture Supernates (100 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC111，測定時間：3.5 hours，測定因子：Leptin/OB 別名：FLJ94114 Genbank No： 3952 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2021年5月1日号 p.8 ニュース2020年2月1日号 p.5
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| 製品記事 |
Leptin/OB Quantikine/DuoSet ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
- Sensitivity
- 7.8 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human Leptin
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human Leptin Immunoassay is a 3.5 hour solid phase ELISA designed to measure soluble human Leptin in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human Leptin and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant Leptin accurately. Results obtained measuring natural human Leptin showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human Leptin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 64.5 | 146 | 621 | 65.7 | 146 | 581 |
| Standard Deviation | 2.14 | 4.32 | 20 | 3.56 | 6.17 | 20.6 |
| CV% | 3.3 | 3 | 3.2 | 5.4 | 4.2 | 3.5 |
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Recovery
The recovery of Leptin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=5) | 95 | 89-109 |
| Heparin Plasma (n=5) | 90 | 81-100 |
| Citrate Plasma (n=5) | 95 | 87-105 |
| EDTA Plasma (n=5) | 99 | 85-112 |
| Cell Culture Media (n=5) | 98 | 94-102 |
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Linearity
To assess the linearity of the assay, cell culture media samples spiked with high concentrations of Leptin and natural serum/plasma samples were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Leptin/OB
- Aliases
- LEP; OB
- Entrez Gene IDs
- 3952 (Human); 16846 (Mouse); 25608 (Rat)
- Background
- Leptin
- Leptin is a product of the mouse obese gene. Mice with mutations in the obese gene that block the synthesis of leptin are obese and diabetic, and have reduced activity, metabolism, and body temperature. Rat leptin shares approximately 96% and 82% sequence identity with the mouse and human protein, respectively. The expression of leptin mRNA is restricted to adipose tissue.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Leptin is associated with vascular endothelial function in overweight patients with type 2 diabetes.
Emoto M, Fukumoto S, Imamura S, Inaba M, Kawano N, Koyama H, Mori K, Morioka T, Motoyama K, Numaguchi R, Shoji T, Urata H, Yamazaki Y
Cardiovasc Diabetol 2014 13:10
Species: Human
Sample Type: Plasma
Alterations of leptin in the course of inflammation and severe sepsis.
BMC Infect Dis 2012 12:217
Species: Human
Sample Type: Serum
A prospective study of circulating adipokine levels and risk of multiple myeloma.
Andreotti G, Baris D, Hofmann J, Lan Q, Landgren O, Liao L, Pfeiffer R, Pollak M, Purdue M, Rothman N, Wang Y
Blood 2012 120:4418-20
Species: Human
Sample Type: Plasma
Adipocytokines and CD34 progenitor cells in Alzheimer's disease.
Bigalke B, Gawaz M, Laske C, Paul A, Schreitmuller B, Sopova K, Stellos K, Stransky E
PLoS ONE 2011 6:e20286
Species: Human
Sample Type: Plasma
Effects of dietary fat modification on insulin sensitivity and on other risk factors of the metabolic syndrome-LIPGENE: a European randomized dietary intervention study.
Blaak EE, Defoort C, Dembinska-Kiec A, Drevon CA, Gibney MJ, Golabek-Leszczynska I, Gulseth HL, Helal O, Karsltrom B, Lopez-Miranda J, Lovegrove JA, McMonagle J, Paniagua JA, Roche HM, Saris WH, Shaw DI, Tierney AC, Vessby B, Williams CM
Int J Obes (Lond) 2011 35:800-9
Species: Human
Sample Type: Serum
Leptin exacerbates sepsis-mediated morbidity and mortality.
Khankin EV, Shapiro NI, van Meurs M
J. Immunol. 2010 185:517-24
Species: Human
Sample Type: Plasma
Exenatide affects circulating cardiovascular risk biomarkers independently of changes in body composition.
Bunck MC, Corner A, Diamant M, Eliasson B, Heine RJ, Shaginian RM, Smith U, Taskinen MR, Yki-Jarvinen H
Diabetes Care 2010 33:1734-7
Species: Human
Sample Type: Serum
Improvements in coronary heart disease risk indicators by alternate-day fasting involve adipose tissue modulations.
Berger RA, Bhutani S, Klempel MC, Varady KA
Obesity (Silver Spring) 2010 18:2152-9
Species: Human
Sample Type: Plasma
Differential adaptation of human gut microbiota to bariatric surgery-induced weight loss: links with metabolic and low-grade inflammation markers.
Basdevant A, Bouillot JL, Clement K, Corthier G, Dore J, Furet JP, Henegar C, Kong LC, Mariat D, Poitou C, Rizkalla S, Tap J
Diabetes 2010 59:3049-57
Species: Human
Sample Type: Plasma
HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
, Ananworanich J, Burger F, Calmy A, Carr A, Gayet-Ageron A, Hirschel B, Mach F, Montecucco F, Nguyen A, Ruxungtham K, Ubolyam S
AIDS 2009 23:929-39
Species: Human
Sample Type: Plasma
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