Human DLL1 Quantikine ELISA Kit | hDLL1 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111138
R&D Systems製のHuman DLL1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月25日 19時55分現在]
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[在庫・価格 ：2025年04月25日 19時55分現在]
Human DLL1 Quantikine ELISA Kit
文献数： 0
- 商品コード：DLL10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：17.4 pg/mL，測定範囲：78.1-5,000 pg/mL，種交差性：Human，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Human Milk，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC77，測定時間：4.5 hours，測定因子：DLL1 別名：Delta Genbank No： 28514 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | R&D Systems社 NotchリガンドDLL1の関連製品 ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL), Human Milk (50 µL)
- Sensitivity
- 17.4 pg/mL
- Assay Range
- 78.1 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human DLL10
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human DLL1 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human DLL1 in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human DLL1 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human DLL1 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human DLL1.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 638 | 1,468 | 2,934 | 592 | 1,340 | 2,714 |
| Standard Deviation | 16 | 38.1 | 76.1 | 42.4 | 85.7 | 189 |
| CV% | 2.5 | 2.6 | 2.6 | 7.2 | 6.4 | 7 |
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Recovery
The recovery of DLL1 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 102 | 96-108 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of DLL1 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- DLL1
- Aliases
- Delta 1
- Entrez Gene IDs
- 28514 (Human); 13388 (Mouse); 84010 (Rat)
- Background
- DLL1
- Delta-like protein 1 (DLL1) is a 90 - 100 kDa type I transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. Mature human DLL1 consists of a 528 amino acid (aa) extracellular domain (ECD) with one DSL domain and eight EGF-like repeats, a 23 aa transmembrane segment, and a 155 aa cytoplasmic domain. Within the ECD, human DLL1 shares 91% aa sequence identity with mouse and rat DLL1. It shares 26%, 37%, and 54% aa sequence identity with DLL2, 3, and 4, respectively. A 60 kDa ECD fragment released by ADAM9, 12, or 17 mediated proteolysis, promotes the proliferation of hematopoietic progenitor cells. The residual membrane-bound portion of DLL1 can be cleaved by presenilin-dependent gamma-secretase, enabling the cytoplasmic domain to migrate to the nucleus.DLL1 localizes to adherens junctions on neuronal processes through its association with the scaffolding protein MAGI1. DLL1 is widely expressed, and it plays an important role in embryonic somite formation, cochlear hair cell differentiation, plus B and T lymphocyte differentiation. The upregulation of DLL1 in arterial endothelial cells following injury or angiogenic stimulation is central to postnatal arteriogenesis. DLL1 is also overexpressed in cervical carcinoma and glioma and contributes to tumor progression.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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