Human LDL R Quantikine ELISA Kit | hLDL R QKit
掲載日情報：2016/08/18 現在Webページ番号：111135
R&D Systems製のHuman LDL Rを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 12時55分現在]
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[在庫・価格 ：2025年04月26日 12時55分現在]
Human LDL R Quantikine ELISA Kit
文献数： 3
- 商品コード：DLDLR0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：10.3 pg/mL，測定範囲：62.5-4,000 pg/mL，種交差性：Human，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Tissue Lysate，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，測定時間：4.5 hours，測定因子：LDLR 別名：FH Genbank No： 3949 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Human / Mouse LDL Receptor ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), Heparin Plasma (10 µL), Urine (50 µL)
- Sensitivity
- 10.3 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, Urine)
- Specificity
- Natural and recombinant human LDL R. This assay also recognizes recombinant human LDL R/PCSK9 complex.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human LDL R Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human LDL R in cell culture supernates, cell lysates, serum, heparin plasma, and urine. It contains NS0-expressed recombinant human LDL R and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human LDL R showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human LDL R.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 260 | 787 | 1,598 | 287 | 823 | 1,602 |
| Standard Deviation | 5.68 | 17.5 | 32.4 | 15.6 | 40 | 74.4 |
| CV% | 2.2 | 2.2 | 2 | 5.4 | 4.9 | 4.6 |
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Recovery
The recovery of human LDL R spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 98 | 92-106 |
| Urine (n=4) | 94 | 91-99 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human LDL R were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- LDL R
- Aliases
- undefined
- Entrez Gene IDs
- 3949 (Human); 16835 (Mouse); 300438 (Rat)
- Background
- LDL R
- The low density lipoprotein receptor (LDLR) is the founding member of the LDLR family of scavenger receptors. This family contains transmembrane molecules that are characterized by the presence of EGF repeats, complement-like repeats, and YWTD motifs that form beta-propellers. Although members of the family were originally thought to be endocytic receptors, it is now clear that some members interact with adjacent cell-surface molecules, expanding their range of activities. Human LDLR is synthesized as an 860 amino acid (aa) precursor that contains a 21 aa signal sequence, a 767 aa extracellular region, a 22 aa transmembrane segment and a 50 aa cytoplasmic tail. The extracellular region is complex. It consists of seven N-terminal complement-like cysteine-rich repeats that bind ligand. Cysteine residues in this region participate in intrachain disulfide bonds. This region is followed by three EGF-like repeats with a beta-propeller YWTD containing motif. The EGF-like repeats are responsible for ligand bonding and dissociation. Finally, there is a 50 aa membrane proximal Ser/Thr-rich region that serves as a carbohydrate attachment point. There is extensive O-linked and modest N-linked glycosylation. Thus the receptor's predicted molecular weight of 93 kDa is increased to a native molecular weight of 120 - 160 kDa. Within the 50 aa cytoplasmic tail, there is an NPXY motif that links the receptor to clathrin pits. The extracellular region of human LDLR is 51% aa identical to the extracellular region of human VLDLR, and 79% aa identical to the extracellular region of mouse LDL R. LDLR is constitutively expressed and binds apoB of LDL and apoE of VLDL. It is responsible for clearing 70% of plasma LDL in liver. Mutations in the LDLR gene cause the autosomal dominant disorder, familial hypercholesterolemia.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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