Human Lipocalin-2/NGAL Quantikine ELISA Kit | hLipocalin-2/NGAL QKit
掲載日情報：2016/08/18 現在Webページ番号：111134
R&D Systems製のHuman Lipocalin-2/NGALを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年07月17日 10時15分現在]
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Lipocalin-2, Human, ELISA Kit, Quantikine (96 well) <NGAL, ELISA Kit> |
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[在庫・価格 ：2025年07月17日 10時15分現在]
Lipocalin-2, Human, ELISA Kit, Quantikine (96 well) <NGAL, ELISA Kit>
文献数： 58
- 商品コード：DLCN20
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.156-10 ng/mL，感度：0.04 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Saliva，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC115，測定時間：4.5 hours，測定因子：Lipocalin-2, NGAL 別名：24p3 Genbank No： 3934 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2017年10月15日号 p.3
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| 製品記事 | Quantikine Human Lipocalin-2 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), Heparin Plasma (50 µL), Saliva (50 µL), Urine (50 µL)
- Sensitivity
- 0.04 ng/mL
- Assay Range
- 0.156 - 10 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Urine)
- Specificity
- Natural and recombinant human Lipocalin-2
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Lipocalin-2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Lipocalin-2 in cell culture supernates, serum, plasma, urine, and saliva. It contains NS0-expressed recombinant human Lipocalin-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human Lipocalin-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Lipocalin-2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 1.14 | 3.45 | 7.54 | 1.05 | 3.37 | 6.92 |
| Standard Deviation | 0.041 | 0.107 | 0.334 | 0.083 | 0.204 | 0.387 |
| CV% | 3.6 | 3.1 | 4.4 | 7.9 | 6.1 | 5.6 |
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Recovery
The recovery of Lipocalin-2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Urine (n=4) | 90 | 84-97 |
| Cell Culture Media (n=4) | 93 | 88-101 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of Lipocalin-2 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Lipocalin-2/NGAL
- Aliases
- LCN2; NGAL; Siderocalin
- Entrez Gene IDs
- 3934 (Human); 16819 (Mouse); 170496 (Rat)
- Background
- Lipocalins
- The Lipocalin family comprises a diverse group of mostly secreted soluble proteins that bind hydrophobic ligands and act as transporters, carrying small molecules to specific cells. Lipocalins are related by possessing an 8-stranded beta-barrel structure. Lipocalin-1, also named tear lipocalin (TL), von Ebners gland protein (VEG) and tear pre-albumin, binds a large number of hydrophobic molecules and exhibits cysteine proteinase inhibitor and endonuclear activities. Lipocalin-2, also known as neutrophil gelatinase-associated lipocalin (NGAL), is a component of granules in neutrophils from tissues that are normally exposed to microorganisms and is upregulated during inflammation. Lipocalin-2 can form homodimers and can heterodimerize with the neutrophil gelatinase MMP-9.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Urinary L-FABP and its combination with urinary NGAL in early diagnosis of acute kidney injury after cardiac surgery in adult patients.
Biomarkers 2013 18:95-101
Species: Human
Sample Type: Urine
Acute kidney injury biomarkers for patients in a coronary care unit: a prospective cohort study.
Chang CH, Chang MY, Chen TH, Chen YC, Fang JT, Hung CC, Jenq CC, Lin CY, Lin FC, Tian YC, Wen MS, Yang CW
PLoS ONE 2012 7:e32328
Urinary neutrophil gelatinase-associated lipocalin is a potential biomarker for renal damage in patients with systemic lupus erythematosus.
Hsieh SC, Li KJ, Lu MC, Tsai CY, Wu CH, Yang CC, Yu CL
J. Biomed. Biotechnol. 2012 2012:759313
Species: Human
Sample Type: Urine
Prevalence of Chronic Kidney Disease and Progression of Disease Over Time among Patients Enrolled in the Houston West Nile Virus Cohort.
Hause AM, Nolan MS, Podoll AS
PLoS ONE 2012 7:e40374
Species: Human
Sample Type: Urine
Indomethacin reduces glomerular and tubular damage markers but not renal inflammation in chronic kidney disease patients: a post-hoc analysis.
de Borst MH, Gansevoort RT, Laverman GD, Nauta FL, Navis G, Vogt L
PLoS ONE 2012 7:e37957
Urinary neutrophil gelatinase-associated lipocalin and L-type fatty acid binding protein as diagnostic markers of early acute kidney injury after liver transplantation.
Li Y, Xia Q, Zhu M
Biomarkers 2012 17:336-42
Species: Human
Sample Type: Urine
The association between the preoperative serum levels of lipocalin-2 and matrix metalloproteinase-9 (MMP-9) and prognosis of breast cancer.
BMC Cancer 2012 12:193
Species: Human
Sample Type: Serum
Lipocalin 2, the TNF-like receptor TWEAKR and its ligand TWEAK act downstream of NFAT1 to regulate breast cancer cell invasion.
de la Grange P, Fougere M, Gaudineau B, Guaddachi F, Jauliac S, Lemoine F
J Cell Sci 2012 125:4475-86
Isotretinoin increases skin-surface levels of neutrophil gelatinase-associated lipocalin in patients treated for severe acne.
Cong Z, Dispenza MC, Gilliland KL, Lumsden KR, Nelson AM, Thiboutot DM, Zaenglein AL
Br. J. Dermatol. 2011 165:302-10
Species: Human
Sample Type: tape-strip extract
Elevated Levels of Neutrophil Gelatinase-Associated Lipocalin in Bile From Patients With Malignant Pancreatobiliary Disease.
Edwards RJ, Gierula M, Horneffer-van der Sluis VM, Khan SA, Laird F, Taylor-Robinson SD, Thillainayagam AV, Vlavianos P, Wadsworth CA, Westaby D, Zabron AA
Am. J. Gastroenterol. 2011 106:1711-7
Species: Human
Sample Type: Bile
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