Human IL-1 alpha/IL-1F1 Quantikine ELISA Kit | "hIL-1a QKit, 2nd Gen"
掲載日情報：2016/08/18 現在Webページ番号：111130
R&D Systems製のHuman IL-1 alpha/IL-1F1を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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IL-1α, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月27日 00時00分現在]
IL-1α, Human, ELISA Kit, Quantikine (96well)
文献数： 64
- 商品コード：DLA50
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：3.9-250 pg/mL，感度：1 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC01-1，測定時間：3.5 hours or 4.5 hours，測定因子：IL-1 alpha, IL-1F1 別名：Hematopoietin-1 Genbank No： 3552 |
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| 別包装品 | 別包装品あり | ||||||
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| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2019年8月15日号 p.15
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| 製品記事 | がん幹細胞関連因子測定用 Quantikine ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours or 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (200 µL), Serum (200 µL), EDTA Plasma (200 µL), Heparin Plasma (200 µL), Citrate Plasma (200 µL)
- Sensitivity
- 1 pg/mL
- Assay Range
- 3.9 - 250 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Citrate Plasma, EDTA Plasma)
- Specificity
- Natural and recombinant human IL-1 alpha
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human IL-1 alpha Immunoassay is a 3.5 or 4.5 hour solid phase ELISA designed to measure IL-1 alpha in cell culture supernates, serum, and plasma. It contains recombinant human IL-1 alpha and antibodies raised against recombinant human IL-1 alpha and has been shown to accurately quantitate the recombinant factor. Results obtained using natural IL-1 alpha showed linear curves that were parallel to the standard curves obtained using the E. coli-expressed Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1 alpha.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 28.6 | 125 | 185 | 27.9 | 123 | 187 |
| Standard Deviation | 0.4 | 1.4 | 4 | 1.2 | 4.7 | 6.3 |
| CV% | 1.4 | 1.1 | 2.2 | 4.3 | 3.8 | 3.4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 26.9 | 122 | 191 | 20.5 | 60 | 121 |
| Standard Deviation | 0.4 | 4.6 | 6.6 | 1.7 | 3.1 | 5.2 |
| CV% | 1.5 | 3.8 | 3.5 | 8.3 | 5.2 | 4.3 |
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Recovery
The recovery of IL-1 alpha spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=6) | 107 | 74-127 |
| Heparin Plasma (n=6) | 104 | 72-126 |
| Citrate Plasma (n=6) | 97 | 71-119 |
| EDTA Plasma (n=6) | 104 | 84-121 |
| Cell Culture Media (n=4) | 96 | 91-104 |
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Linearity
To assess the linearity of the assay, the following biological samples spiked with high concentrations of IL-1 alpha were diluted with the appropriate Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- IL-1 alpha/IL-1F1
- Aliases
- IL1A; IL-1F1
- Entrez Gene IDs
- 3552 (Human); 16175 (Mouse); 24493 (Rat); 397094 (Porcine)
- Background
- IL-1 alpha/IL-1F1
- Interleukin-1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL 4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Recent evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Role of IL-1alpha and the Nlrp3/caspase-1/IL-1beta axis in cigarette smoke-induced pulmonary inflammation and COPD.
Bracke KR, Brusselle GG, Dupont LL, Joos GF, Lambrecht BN, Pauwels NS, Provoost S, Van Pottelberge GR, Vanden Berghe T, Vandenabeele P
Eur. Respir. J. 2011 38:1019-28
Species: Human
Sample Type: Sputum
HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
Brandt O, Elbe-Burger A, Eppel W, Fiala C, Imhof M, Meindl S, Schuster C, Stingl G, Vaculik C
J. Exp. Med. 2009 206:169-81
Species: Human
Sample Type: Cell Culture Supernates
In vivo regulation of interleukin 1beta in patients with cryopyrin-associated periodic syndromes.
Bek S, Bosset S, Felix SD, Hartmann N, Hawkins PN, Jung T, Lachmann HJ, Leslie K, Lowe P, Madhoo S, Rordorf C, Wittkowski H
J. Exp. Med. 2009 206:1029-36
Species: Human
Sample Type: Plasma
RAS mutations affect tumor necrosis factor-induced apoptosis in colon carcinoma cells via ERK-modulatory negative and positive feedback circuits along with non-ERK pathway effects.
Alford SK, Haigis KM, Kreeger PK, Lauffenburger DA, Mandhana R
Cancer Res. 2009 69:8191-9
Species: Human
Sample Type: Cell Culture Supernates
Selective inhibition of proprotein convertases represses the metastatic potential of human colorectal tumor cells.
Basak A, Bontemps Y, Calvo F, Cherel G, Khatib AM, Ma L, Scamuffa N, Seidah NG, Siegfried G
J. Clin. Invest. 2008 118:352-63
Species: Human
Sample Type: Cell Culture Supernates
Peroxisome proliferator-activated receptor-gamma agonists inhibit respiratory syncytial virus-induced expression of intercellular adhesion molecule-1 in human lung epithelial cells.
Arnold R, Konig W, Neumann M
Immunology 2007 121:71-81
Species: Human
Sample Type: Cell Culture Supernates
Administration of cyclooxygenase-2 inhibitor reduces joint inflammation but exacerbates osteopenia in IL-1 alpha transgenic mice due to GM-CSF overproduction.
Kosaki N, Matsumoto H, Miyamoto T, Niki Y, Tada N, Takaishi H, Takito J, Toyama Y
J. Immunol. 2007 179:639-46
Species: Human
Sample Type: Cell Culture Supernates
The influence of anger expression on wound healing.
Glaser R, Gouin JP, Kiecolt-Glaser JK, Malarkey WB
Brain Behav. Immun. 2007 22:699-708
Species: Human
Sample Type: Wound Secretion
Protection of human keratinocytes from UVB-induced inflammation using root extract of Lithospermum erythrorhizon.
, Ishida T, Sakaguchi I
Biol. Pharm. Bull. 2007 30:928-34
Species: Human
Sample Type: Cell Culture Supernates
The response of human epithelial cells to TNF involves an inducible autocrine cascade.
Albeck JG, Gaudet S, Janes KA, Lauffenburger DA, Nielsen UB, Sorger PK
Cell 2006 124:1225-39
Species: Human
Sample Type: Cell Culture Supernates
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