Human Kallikrein 5 Quantikine ELISA Kit | hKLK5 QKit
掲載日情報：2016/08/18 現在Webページ番号：111127
R&D Systems製のHuman Kallikrein 5を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 08時35分現在]
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Kallikrein 5, Human, ELISA Kit, Quantikine (96well) <KLK5, ELISA Kit> |
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[在庫・価格 ：2025年04月26日 08時35分現在]
Kallikrein 5, Human, ELISA Kit, Quantikine (96well) <KLK5, ELISA Kit>
文献数： 1
- 商品コード：DKK500
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：78.1-5,000 pg/mL，感度：30 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Saliva, Human Milk，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC109，測定時間：4.5 hours，測定因子：Kallikrein 5 別名：EC 3.4.21 Genbank No： 25818 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Human Kallikrein 5 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Saliva (13 µL), Human Milk (13 µL)
- Sensitivity
- 30 pg/mL
- Assay Range
- 78.1 - 5,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma)
- Specificity
- Natural and recombinant human KLK5
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human KLK5 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human KLK5 in cell culture supernates, serum, plasma, saliva, and human milk. It contains NS0-expressed recombinant human KLK5 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human KLK5 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human KLK5.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 510 | 1,685 | 3,407 | 577 | 1,941 | 3,852 |
| Standard Deviation | 35.8 | 57.2 | 75.4 | 63.6 | 161 | 278 |
| CV% | 7 | 3.4 | 2.2 | 11 | 8.3 | 7.2 |
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Recovery
The recovery of KLK5 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 97 | 91-109 |
| Serum (n=4) | 93 | 85-106 |
| EDTA Plasma (n=4) | 90 | 82-95 |
| Heparin Plasma (n=4) | 91 | 86-103 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of KLK5 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Kallikrein 5
- Aliases
- KLK5; KLK-L2; SCTE
- Entrez Gene IDs
- 25818 (Human); 68668 (Mouse); 690603 (Rat)
- Background
- Kallikreins
- The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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