Human Kallikrein 3/PSA Quantikine ELISA Kit | hKLK3 QKit
掲載日情報：2016/08/18 現在Webページ番号：111126
R&D Systems製のHuman Kallikrein 3/PSAを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 08時35分現在]
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Kallikrein 3, Human, ELISA Kit, Quantikine (96well) <KLK3, ELISA Kit><PSA, ELISA Kit> |
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[在庫・価格 ：2025年04月26日 08時35分現在]
Kallikrein 3, Human, ELISA Kit, Quantikine (96well) <KLK3, ELISA Kit><PSA, ELISA Kit>
文献数： 14
- 商品コード：DKK300
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：0.94-60 ng/mL，感度：0.069 ng/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC23，測定時間：4.5 hours，測定因子：Kallikrein 3, PSA 別名：APS seminin Genbank No： 354 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Human Kallikrein 5 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- undefined
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 0.069 ng/mL
- Assay Range
- 0.94 - 60 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human KLK3/PSA
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human KLK3/PSA Immunoassay is a 4.5 hour solid phase immunoassay designed to measure KLK3/PSA in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human KLK3/PSA, and antibodies raised against the recombinant protein. Natural human KLK3/PSA showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human KLK3/PSA.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 12.3 | 24.3 | 44.1 | 11.8 | 24.2 | 43.8 |
| Standard Deviation | 0.7 | 1.3 | 2 | 0.9 | 0.5 | 0.8 |
| CV% | 5.7 | 5.4 | 4.6 | 7.7 | 5.8 | 5.5 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 6.96 | 15.5 | 30 | 7.38 | 16.7 | 33 |
| Standard Deviation | 0.5 | 0.7 | 0.9 | 0.5 | 0.9 | 1.6 |
| CV% | 7.8 | 4.5 | 3 | 6.4 | 5.3 | 5 |
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Recovery
The recovery of KLK3/PSA spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 102 | 97-109 |
| Heparin Plasma (n=4) | 101 | 97-109 |
| EDTA Plasma (n=4) | 100 | 93-103 |
| Cell Culture Media (n=4) | 99 | 93-105 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of KLK3/PSA were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Kallikrein 3/PSA
- Aliases
- KLK3; PSA
- Entrez Gene IDs
- 354 (Human)
- Background
- Kallikreins
- The human tissue kallikrein (KLK) gene family contains 15 members that play important roles in cancer. Notably, kallikrein-1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. Kallikrein-3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. Kallikrein-4 is specifically expressed in the prostate and over-expressed in prostate cancer. Kallikrein-5 is widely expressed but found at high levels in skin, breast, brain and testis; over-expression is an indicator of poor prognosis in ovarian cancer. Kallikrein-8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.Human plasma kallikrein, a serine protease, is synthesized in the liver and circulates in the plasma bound to high molecular weight (HMW) kininogen or as a free zymogen. Once activated by its physiological activator, coagulation factor XII, it displays endopeptidase activity towards peptide bonds after arginine (preferred) and lysine. It cleaves HMW kininogen, its major physiological substrate, to release the potent vasodilator peptide bradykinin. It is also able to cleave a number of inactive precursor proteins to generate active products, such as plasminogen and prourokinase.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Antitumor activity of a pyrrole-imidazole polyamide.
Proc Natl Acad Sci U S A 2013 110:1863-8
Species: Human
Sample Type: Serum
Synergistic activity of the mTOR inhibitor ridaforolimus and the antiandrogen bicalutamide in prostate cancer models.
Clackson T, Miller D, Rivera VM, Squillace RM, Wang F, Wardwell SD
Int. J. Oncol. 2012 41:425-32
Species: Human
Sample Type: Cell Culture Supernates
In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
Avery V, Glover T, Kiss D, Windus L
Exp Cell Res 2012 318:2507-19
Species: Human
Sample Type: Cell Culture Supernates
Metformin enhances the antiproliferative and apoptotic effect of bicalutamide in prostate cancer.
Besla , R, Colquhoun , A J, Fleshner , N E, Kiss , A, Klotz , L H, Pollak , M, Sugar , L M, Vandersluis , A D, Venier , N A, Venkateswaran , V
Prostate Cancer Prostatic Dis 2012 15:346-52
Species: Mouse
Sample Type: Serum
Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
Alderborn A, Darmanis S, Ericsson O, Gu J, Gustafsdottir S, Hammond M, Kamali-Moghaddam M, Landegren U, Nong RY, Siegbahn A, Vanelid J
Mol. Cell Proteomics 2010 9:327-35
Species: Human
Sample Type: Plasma
D,L-Sulforaphane causes transcriptional repression of androgen receptor in human prostate cancer cells.
Kim SH, Singh SV
Mol. Cancer Ther. 2009 8:1946-54
Species: Human
Sample Type: Cell Culture Supernates
Transcriptional repression and inhibition of nuclear translocation of androgen receptor by diallyl trisulfide in human prostate cancer cells.
Singh SV, Stan SD
Clin. Cancer Res. 2009 15:4895-903
Species: Human
Sample Type: Cell Culture Supernates
Urine analysis and protein networking identify met as a marker of metastatic prostate cancer.
Albert PS, Bottaro DP, Camphausen K, Citrin D, Dahut W, Figg WD, Jedlicka K, Kaushal A, Kirk M, McNally D, Russo AL, Shankavaram U, Smith S, Sproull M, Tofilon PJ, Wernick M
Clin. Cancer Res. 2009 15:4292-8
Species: Human
Sample Type: Cell Culture Supernates
Isosilybin B causes androgen receptor degradation in human prostate carcinoma cells via PI3K-Akt-Mdm2-mediated pathway.
Agarwal R, Deep G, Kroll DJ, Oberlies NH
Oncogene 2008 27:3986-98
Species: Human
Sample Type: Cell Culture Supernates
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