Human/Canine/Porcine Insulin Quantikine ELISA Kit | h/c/pInsulin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111122
R&D Systems製のHuman/Canine/Porcine Insulinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月22日 20時15分現在]
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Insulin, Human/Canine/Porcine, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2024年05月22日 20時15分現在]
Insulin, Human/Canine/Porcine, ELISA Kit, Quantikine (96well)
文献数： 10
- 商品コード：DINS00
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
測定範囲：15.60 - 500 pmol/L，感度：2.15 pmol/L，測定試料：Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL), Heparin Plasma (50 uL)，種交差性：Human/Porcine/Canine，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC107，測定時間：4.5 hours，測定因子：Insulin 別名：IDDM2 Genbank No： 3630 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Insulin ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL)
- Sensitivity
- 2.15 pmol/L
- Assay Range
- 15.6 - 500 pmol/L (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Recombinant human Insulin and natural human, canine, and porcine Insulin.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human/Canine/Porcine Insulin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure Insulin in cell culture supernates, serum, and plasma. It contains S. cerevisiae-expressed recombinant human Insulin and has been shown to accurately quantitate the recombinant factor. Results obtained using natural Insulin showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring Insulin.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 66.8 | 158 | 299 | 62.2 | 148 | 288 |
| Standard Deviation | 2.58 | 5.89 | 11.9 | 4.6 | 10.2 | 21.5 |
| CV% | 3.9 | 3.7 | 4 | 7.4 | 6.9 | 7.5 |
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Recovery
The recovery of Insulin spiked to levels throughout the range of the assay in various matrices was evaluated
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 95 | 85-108 |
| Serum (n=4) | 96 | 87-103 |
| EDTA Plasma (n=4) | 95 | 86-102 |
| Heparin Plasma (n=4) | 92 | 85-105 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of Insulin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Insulin
- Aliases
- IDDM2; ILPR; INS; IRDN; MODY10
- Entrez Gene IDs
- 3630 (Human); 16333 (Mouse); 280829 (Bovine)
- Background
- Insulin
- Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. Insulin processes out part of the G, leaving two parallel, but disulfide linked segments; the IGFs only process at the ends, retaining the basic configuration.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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