Human Insulin C-Peptide Quantikine ELISA Kit | hInsulin C-Peptide Qkit

掲載日情報:2016/08/18 現在Webページ番号:111120

R&D Systems製のHuman Insulin C-Peptideを測定するELISAキットです。
本製品は研究用です。研究用以外には使用できません。

[在庫・価格 :2025年04月26日 00時00分現在]

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Insulin C-Peptide, Human, ELISA Kit, Quantikine
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説明文
測定試料:Serum, Plasma, Cell Culture Supernatant, Urine,測定範囲:28.9-1,850 pmol/L,感度:2.88 pmol/L,測定波長:450 nm(補正波長:540 nmまたは570 nm),別売品コントロール:#QC108,種交差性:Human,アッセイ法:Solid Phase Sandwich ELISA,測定時間:2.5 hours,測定因子:C-Peptide
別名:C-P
Genbank No: 3630
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Human C-Peptide Quantikine/DuoSet ELISA Kit
ELISA Kit製品に関するFAQ(R&D Systems)
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集
Quantikine ELISA Kit

[在庫・価格 :2025年04月26日 00時00分現在]

※ 表示されている納期は弊社に在庫が無く、取り寄せた場合の納期目安となります。

Insulin C-Peptide, Human, ELISA Kit, Quantikine

文献数: 1

説明文 測定試料:Serum, Plasma, Cell Culture Supernatant, Urine,測定範囲:28.9-1,850 pmol/L,感度:2.88 pmol/L,測定波長:450 nm(補正波長:540 nmまたは570 nm),別売品コントロール:#QC108,種交差性:Human,アッセイ法:Solid Phase Sandwich ELISA,測定時間:2.5 hours,測定因子:C-Peptide
別名:C-P
Genbank No: 3630
法規制等
保存条件 4℃ 法規備考
掲載カタログ

製品記事 Human C-Peptide Quantikine/DuoSet ELISA Kit
ELISA Kit製品に関するFAQ(R&D Systems)
関連記事 R&D Systems Quantikine ELISA Kit/DuoSet Kit特集
Quantikine ELISA Kit

Product Details

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
2.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 µL), Serum (50 µL), EDTA Plasma (50 µL), Heparin Plasma (50 µL), Urine (10 µL)

Sensitivity
2.88 pmol/L
Assay Range
28.9 - 1,850 pmol/L (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
Specificity
Natural and synthetic human Insulin C-Peptide.
Interference
No significant interference observed with available related molecules.

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Product Summary

The Quantikine Human Insulin C-Peptide Immunoassay is a 2.5 hour solid-phase ELISA designed to measure human Insulin C-Peptide in cell culture supernates, serum, plasma, and urine. It contains synthetic human Insulin C-Peptide and has been shown to accurately quantitate the peptide. Results obtained using natural human Insulin C-Peptide showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human Insulin C-Peptide.

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Precision

Intra-Assay Precision (Precision within an assay)
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean 129 436 845 127 437 861
Standard Deviation 5.77 14.3 13.2 12.4 33 65.3
CV% 4.5 3.3 1.6 9.8 7.6 7.6

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Recovery

The recovery of human Insulin C-Peptide spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 99 84-106
Serum (n=4) 103 96-109
EDTA Plasma (n=4) 101 95-108
Heparin Plasma (n=4) 102 90-115
Urine (n=4) 97 81-106

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Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Insulin C-Peptide were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.

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Additional Infomation

Molecule Information
Insulin
Aliases
IDDM2; ILPR; INS; IRDN; MODY10
Entrez Gene IDs
3630 (Human); 16333 (Mouse); 280829 (Bovine)
Background
Insulin
Insulin and the IGFs are members of the insulin family of molecules. IGF-I and II are structurally homologous to proinsulin, and can be thought of as taking the shape of an exaggerated, inverted letter G. Intrachain disulfide bonds link sections of the G. Insulin processes out part of the G, leaving two parallel, but disulfide linked segments; the IGFs only process at the ends, retaining the basic configuration.

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Citations

Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.

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お問い合わせ先

(テクニカルサポート 試薬担当)

reagent@funakoshi.co.jp

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