Hyaluronan Quantikine ELISA Kit | Hyaluronan Qkit
掲載日情報：2016/08/18 現在Webページ番号：111119
R&D Systems製のHyaluronanを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月29日 20時55分現在]
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[在庫・価格 ：2025年04月29日 20時55分現在]
Hyaluronan Quantikine ELISA Kit
文献数： 41
- 商品コード：DHYAL0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | ELISA, キット, hyaluronan, kit, hyaluronic acid, ヒアルロン酸, ヒアルロン, ヒアルロン酸ELISAキット。感度：0.2 ng/mL，測定範囲：0.625-40 ng/mL，種交差性：Multi-species，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC104，測定時間：4.5 hours，測定因子：Hyaluronan |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2021年11月15日号 p.18
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| 製品記事 | Quantikine Hyaluronan ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Tissue Homogenates (50 µL), Serum (25 µL), EDTA Plasma (25 µL), Heparin Plasma (25 µL)
- Sensitivity
- 0.2 ng/mL
- Assay Range
- 0.625 - 40 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates)
- Specificity
- Measures greater than or equal to 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine samples
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Hyaluronan Immunoassay is a 4.5 hour solid-phase ELISA designed to measure at least 35 kDa Hyaluronan in human, mouse, rat, canine, and porcine cell culture supernates, tissue homogenates, serum, and plasma samples. It contains Streptococcus pyogenes-derived Hyaluronan and NS0-expressed human Aggrecan. This immunoassay has been shown to accurately quantitate Hyaluronan.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.53 | 4.81 | 14.3 | 1.68 | 4.68 | 14.98 |
| Standard Deviation | 0.11 | 0.26 | 0.52 | 0.08 | 0.23 | 0.72 |
| CV% | 7.2 | 5.4 | 3.6 | 4.8 | 4.9 | 4.8 |
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Recovery
The recovery of Hyaluronan spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=2) | 104 | 96-116 |
| Serum (n=10) | 98 | 81-119 |
| EDTA Plasma (n=10) | 97 | 83-106 |
| Heparin Plasma (n=10) | 99 | 80-124 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of Hyaluronan were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Hyaluronan
- Aliases
- undefined
- Entrez Gene IDs
- undefined
- Background
- Hyaluronan
- Hyaluronan, also known as hyaluronic acid (HA) or sodium hyaluronate, is a naturally occurring linear polymer. Hyaluronan is a glycosaminoglycan (GAG) that is ubiquitously present in the extracellular matrix of all vertebrates and is also present in the capsule of some strains of Streptococci. Mammalian hyaluronan is synthesized by one of three distinct hyaluronan synthases (HAS1, 2, and 3), which produce HA polymers with different chain lengths and differ in their rates of synthesis. Whereas high molecular weight HA (> 500 kDa) is anti-angiogenic, anti-inflammatory and immunosuppressive, low molecular weight HA (10 - 500 kDa) is highly angiogenic and pro-inflammatory. HA oligomers are anti-apoptotic and upregulate heat shock protein expression. Functionally, hyaluronan molecules are important for the maintenance of a highly hydrated extracellular matrix in tissues, which is involved in cell adhesion and supports cell migration.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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