Human ErbB2/Her2 Quantikine ELISA Kit | hErbB2/Her2 QKit
掲載日情報：2016/08/18 現在Webページ番号：111115
R&D Systems製のHuman ErbB2/Her2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Human ErbB2/Her2 Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Human ErbB2/Her2 Quantikine ELISA Kit
文献数： 1
- 商品コード：DHER20
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：78.1-5,000 pg/mL，感度：14.8 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine, Tissue Lysate, Human Milk，種交差性：Human，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC86，測定時間：4.5 hours，測定因子：ErbB2, Her2 別名：CD340 Genbank No： 2064 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2018年7月1日号 p.11
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| 製品記事 | がん幹細胞関連因子測定用 Quantikine ELISA Kit Quantikine ErbB2 / Her2 Human ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (50 µL), Human Milk (50 µL)
- Sensitivity
- 14.8 pg/mL
- Assay Range
- 78.1 - 5,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Human Milk, Cell Lysates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human ErbB2.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human ErbB2/Her2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human ErbB2 in cell culture supernates, cell lysates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human ErbB2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human ErbB2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human ErbB2.
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Precision
Intra-Assay Precision (Precision within an assay)
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 471 | 1,379 | 2,681 | 489 | 1,419 | 2,667 |
| Standard Deviation | 6.71 | 16.3 | 31.1 | 18.7 | 56.4 | 93.5 |
| CV% | 1.4 | 1.2 | 1.2 | 3.8 | 4 | 3.5 |
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Recovery
The recovery of human ErbB2 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 103 | 90-120 |
| Serum (n=4) | 99 | 91-115 |
| EDTA Plasma (n=4) | 99 | 89-118 |
| Heparin Plasma (n=4) | 104 | 92-119 |
| Urine (n=4) | 101 | 94-106 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human ErbB2 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- ErbB2/Her2
- Aliases
- CD340; HER2; Neu Oncogene; NGL; TKR1
- Entrez Gene IDs
- 2064 (Human); 13866 (Mouse); 24337 (Rat)
- Background
- ErbB2
- ErbB2, also known as Neu and Her2, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the EGF family of growth factors. Among ErbB family members, ErbB2 is unique in that it has no identified ligands. Rather, ErbB2 heterodimerizes with the other ErbB family members (EGF R, ErbB3, and ErbB4) to form higher affinity signaling complexes.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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