Human PDGF-AB Quantikine ELISA Kit | hPDGF-AB Qkit
掲載日情報：2016/08/18 現在Webページ番号：111113
R&D Systems製のHuman PDGF-ABを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年07月20日 00時00分現在]
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Human PDGF-AB Quantikine ELISA Kit |
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[在庫・価格 ：2025年07月20日 00時00分現在]
Human PDGF-AB Quantikine ELISA Kit
文献数： 37
- 商品コード：DHD00C
- メーカー：RSD
- 包装：1kit
- 価格：¥98,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 種交差性：Human，感度：3.83 pg/mL，測定範囲：15.6-1,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，測定波長：450 nm，検出方法：呈色，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC20，測定時間：4.5 hours，測定因子：PDGF-AB Genbank No： 5154 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (10 µL), Platelet-poor EDTA Plasma (100 µL), Platelet-poor Heparin Plasma (100 µL)
- Sensitivity
- 3.83 pg/mL
- Assay Range
- 15.6 - 1,000 pg/mL (Serum, Cell Culture Supernates, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
- Specificity
- Natural and recombinant human PDGF-AB
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine PDGF-AB Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-AB in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-AB and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-AB showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-AB.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 92.7 | 312 | 656 | 102 | 343 | 701 |
| Standard Deviation | 8.28 | 9.73 | 15.2 | 10.9 | 29.9 | 52.3 |
| CV% | 8.9 | 3.1 | 2.3 | 10.7 | 8.7 | 7.5 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 107 | 350 | 726 | 119 | 362 | 723 |
| Standard Deviation | 6.95 | 10.9 | 63.1 | 10.7 | 20.9 | 54.5 |
| CV% | 6.5 | 3.1 | 8.7 | 9 | 5.8 | 7.5 |
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Recovery
The recovery of PDGF-AB spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 105 | 93-119 |
| Platelet-poor EDTA Plasma (n=4) | 104 | 78-117 |
| Platelet-poor Heparin Plasma (n=4) | 105 | 86-121 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-AB were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- PDGF-AB
- Aliases
- undefined
- Entrez Gene IDs
- undefined
- Background
- PDGF-A, -B, and -D
- The platelet-derived growth factor (PDGF) family of disulfide-linked dimeric proteins consists of four homodimeric proteins, PDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD, and one heterodimeric protein, PDGF-AB. Members of this protein family act mainly on connective tissue. The dimeric isoforms of PDGF are expressed by different cell types and at different times during embryonic development. All PDGF isoforms are synthesized as inactive precursors and are processed to active forms that contain a PDGF/VEGF cysteine knot domain. Only PDGF-C and PDGF-D, also named spinal cord-derived growth factors SCDGF and SCDGF-B, also contain an N-terminal CUB domain. Mature PDGF dimers bind and induce the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta).
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Bieback K, Dietz L, Kinzebach S, Kluter H, Thierse H
BMC Cell Biol 2013 14:48
Species: Human
Sample Type: Cell Lysates
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Amann EM, Feifel E, Gstraunthaler G, Pfaller W, Rauch C, Schennach H, Spotl HP
ALTEX 2011 28:305-16
Species: Human
Sample Type: Cell Lysates
Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
Ding W, Knox TR, Tschumper RC
Blood 2010 116:2984-93
Species: Human
Sample Type: Platelet-Poor Plasma
Oncogenic and angiogenic growth factors accumulate during routine storage of apheresis platelet concentrates.
Gore L, Kanter J, Kelher M, Khan SY, Silliman CC
Clin. Cancer Res. 2008 14:3942-7
Species: Human
Sample Type: Platelet-Poor Plasma
Dynamic change in phosphorylated platelet-derived growth factor receptor in peripheral blood leukocytes following docetaxel therapy predicts progression-free and overall survival in prostate cancer.
Bucana C, Fidler IJ, Horne E, Jones D, Lee YC, Lin SH, Logothetis CJ, Mathew P, Morris MJ, Oh WK, Thall PF, Wen S
Br. J. Cancer 2008 99:1426-32
Species: Human
Sample Type: Plasma
Selective cyclooxygenase-2 inhibitor downregulates the paracrine epithelial-mesenchymal interactions of growth in scirrhous gastric carcinoma.
Hirakawa K, Kosaka K, Nakazawa K, Shinto O, Tendo M, Yashiro M
Int. J. Cancer 2007 120:686-93
Species: Human
Sample Type: Cell Culture Supernates
TF/FVIIa transactivate PDGFRbeta to regulate PDGF-BB-induced chemotaxis in different cell types: involvement of Src and PLC.
Aberg M, Johnell M, Nordin A, Siegbahn A, Velling T
Arterioscler. Thromb. Vasc. Biol. 2007 28:135-41
Species: Porcine
Sample Type: Cell Culture Supernates
Tumor-driven paracrine platelet-derived growth factor receptor alpha signaling is a key determinant of stromal cell recruitment in a model of human lung carcinoma.
Dong J, Ferrara N, Frantz GD, Gerber HP, Hall L, Jung K, Liang X, Meng G, Peale FV, Tejada ML, Yu L
Clin. Cancer Res. 2006 12:2676-88
Species: Human
Sample Type: Cell Culture Supernates
Eosinophil-derived cationic proteins activate the synthesis of remodeling factors by airway epithelial cells.
Gleich GJ, Pegorier S, Pretolani M, Wagner LA
J. Immunol. 2006 177:4861-9
Species: Human
Sample Type: Cell Culture Supernates
Expression of PDGF and their receptors in human retinal pigment epithelial cells and fibroblasts: regulation by TGF-beta.
Detrick B, Hooks JJ, Kutty V, Nagineni CN
J. Cell. Physiol. 2005 203:35-43
Species: Human
Sample Type: Cell Culture Supernates
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