Human Haptoglobin Quantikine ELISA Kit | hHaptoglobin QKit
掲載日情報：2016/08/18 現在Webページ番号：111112
R&D Systems製のHuman Haptoglobinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月03日 00時01分現在]
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Haptoglobin, Human, ELISA Kit, Quantikine |
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[在庫・価格 ：2024年05月03日 00時01分現在]
Haptoglobin, Human, ELISA Kit, Quantikine
文献数： 5
- 商品コード：DHAPG0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：0.529 ng/mL，測定範囲：3.13 - 200 ng/mL，測定試料：Serum-free Cell Culture Supernates (20 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Saliva (10 uL), Urine (20 uL)，測定波長：450 nm，種交差性：Human，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，測定時間：4.5 hours，測定因子：Haptoglobin 別名：binding peptide Genbank No： 3240 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human Haptoglobin ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Serum-free Cell Culture Supernates (20 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Saliva (10 µL), Urine (20 µL)
- Sensitivity
- 0.529 ng/mL
- Assay Range
- 3.13 - 200 ng/mL (Serum, Heparin Plasma, Saliva, EDTA Plasma, Urine, Serum-free Cell Culture Supernates)
- Specificity
- Natural human haptoglobin
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Haptoglobin Immunoassay is a 4.5 hour solid phase ELISA designed to measure haptoglobin in serum-free cell culture supernates, serum, plasma, saliva, and urine
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of a known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in separate assays to determine inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Serum-free Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Saliva, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 2 | 3 | 3 | 1 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 18.7 | 58.3 | 57.8 | 113 | 116 | 18.8 |
| Standard Deviation | 0.69 | 1.42 | 3.47 | 6.3 | 3.66 | 1.4 |
| CV% | 3.7 | 2.4 | 6 | 5.6 | 3.2 | 7.4 |
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Recovery
The recovery of human haptoglobin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum-free Cell Culture Media (n=4) | 101 | 94-107 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human haptoglobin were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. 
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Additional Infomation
- Molecule Information
- Haptoglobin
- Aliases
- HP; HP-1; hp2-alpha; HPA1S; Zonulin
- Entrez Gene IDs
- 3240 (Human); 15439 (Mouse); 24464 (Rat)
- Background
- Haptoglobin
- Haptoglobin is an acute phase glycoprotein that is an inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and binds with high affinity to circulating Hemoglobin. Association of Haptoglobin with free Hemoglobin protects tissues from Hemoglobin-mediated oxidative damage and facilitates Hemoglobin clearance. In addition, Haptoglobin has anti-inflammatory properties that are independent of Hemoglobin binding. It inhibits prostaglandin synthesis, scavenges nitric oxide, dampens the inflammatory response to endotoxins, and inhibits T cell proliferation and neutrophil respiratory bursts. Deletion of Haptoglobin in mice facilitates the development of autoimmune inflammation.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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