GDF-8/Myostatin Quantikine ELISA Kit | GDF-8/Myostatin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111107
R&D Systems製のGDF-8/Myostatinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 20時55分現在]
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GDF-8/Myostatin Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月26日 20時55分現在]
GDF-8/Myostatin Quantikine ELISA Kit
文献数： 37
- 商品コード：DGDF80
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 種交差性：Multi-species，感度：5.32 pg/mL，測定範囲：31.3-2,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC98，測定時間：4.5 hours，測定因子：GDF-8, Myostatin 別名：GDF8 Genbank No： 2660 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2019年11月15日号 p.13
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| 製品記事 | Quantikine GDF-8 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Tissue Homogenates (20 µL), Serum (100 µL), EDTA Plasma (100 µL), Heparin Plasma (100 µL)
- Sensitivity
- 5.32 pg/mL
- Assay Range
- 31.3 - 2,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Tissue Homogenates)
- Specificity
- Natural and recombinant human, mouse, rat, canine, porcine, and equine GDF-8.
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine GDF-8/Myostatin Immunoassay is a 4.5 hour solid phase ELISA designed to measure GDF-8 levels in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant GDF-8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant GDF-8 accurately. Results obtained using natural GDF-8 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural GDF-8.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of a known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of kit components.
Cell Culture Supernates, Tissue Homogenates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 171 | 583 | 1,147 | 146 | 491 | 1,058 |
| Standard Deviation | 9.2 | 14.8 | 20.9 | 8.8 | 17.7 | 32.3 |
| CV% | 5.4 | 2.5 | 1.8 | 6 | 3.6 | 3.1 |
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Recovery
The recovery of GDF-8 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Samples (n=4) | 110 | 100-118 |
| Tissue Homogenates (n=4) | 100 | 90-120 |
| Serum and Plasma (n=12) | 91 | 80-117 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of GDF-8 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- GDF-8/Myostatin
- Aliases
- MSTN; Myostatin
- Entrez Gene IDs
- 2660 (Human); 17700 (Mouse)
- Background
- Growth/Differentiation Factors (GDFs)
- Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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