Human GDF-15 Quantikine ELISA Kit | hGDF-15 QKit
掲載日情報：2016/08/18 現在Webページ番号：111106
R&D Systems製のHuman GDF-15を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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GDF-15, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月27日 00時00分現在]
GDF-15, Human, ELISA Kit, Quantikine (96well)
文献数： 58
- 商品コード：DGD150
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(発注済)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 測定範囲：23.4-1,500 pg/mL，感度：4.39 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC21，測定時間：3.5 hours，測定因子：GDF-15 別名：GDF15 Genbank No： 9518 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
ニュース2025年3月15日号 p.15
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| 製品記事 | Quantikine Human GDF-15 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL), Urine (10 µL)
- Sensitivity
- 4.39 pg/mL
- Assay Range
- 23.4 - 1,500 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human GDF-15
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human GDF-15 immunoassay is a 3.5 hour solid phase ELISA designed to measure GDF-15 in cell culture supernates, serum, plasma, and urine. It contains CHO cell-expressed recombinant human GDF-15 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural GDF-15 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of naturally occurring GDF-15.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 238 | 456 | 886 | 225 | 442 | 900 |
| Standard Deviation | 4.27 | 9.86 | 25 | 13.4 | 20.6 | 50.5 |
| CV% | 1.8 | 2.2 | 2.8 | 6 | 4.7 | 5.6 |
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Recovery
The recovery of GDF-15 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 98 | 88-107 |
| Heparin Plasma (n=4) | 96 | 86-108 |
| EDTA Plasma (n=4) | 97 | 85-108 |
| Urine (n=4) | 102 | 88-108 |
| Cell Culture Media (n=4) | 100 | 94-106 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of GDF-15 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- GDF-15
- Aliases
- MIC-1; NAG-1; PDF; PLAB; PTGF-beta
- Entrez Gene IDs
- 9518 (Human); 23886 (Mouse); 29455 (Rat)
- Background
- Growth/Differentiation Factors (GDFs)
- Growth/differentiation factors (GDF-1 to GDF-15) are members of the BMP family of TGF-beta superfamily proteins. They are produced as inactive preproproteins which are then cleaved and assembled into active secreted homodimers. GDF dimers are disulfide-linked with the exception of GDF-3 and -9. GDF proteins are important during embryonic development, particularly in the skeletal, nervous, and muscular systems.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Hypoxia potentiates glioma-mediated immunosuppression.
Fokt I, Fuller G, Heimberger AB, Kong LY, Melillo G, Priebe W, Wang Y, Wei J, Wu A
PLoS ONE 2012 6:e16195
Species: Human
Sample Type: Cell Culture Supernates
Regulation of hepcidin expression at high altitude.
Dorrington KL, Lakhal S, Leon-Velarde F, Mole DR, Nickol AH, Privat C, Rivera-Ch M, Robbins PA, Smith TG, Talbot NP
Blood 2012 119:857-60
Bone marrow mesenchymal stem cells in patients with beta thalassemia major: molecular analysis with attenuated total reflection-Fourier transform infrared spectroscopy study as a novel method.
Stem Cells Dev. 2012 21:2000-11
The relationship between insulin resistance and the cardiovascular biomarker growth differentiation factor-15 in obese patients.
Anderwald C, Clodi M, Handisurya A, Krebs M, Ludvik B, Luger A, Prager G, Resl M, Riedl M, Vila G
Clin. Chem. 2011 57:309-16
Species: Human
Sample Type: Plasma
Modulation of hepcidin production during hypoxia-induced erythropoiesis in humans in vivo: data from the HIGHCARE project.
Galimberti S, Mariani R, Piperno A
Blood 2011 117:2953-9
Species: Human
Sample Type: Serum
Growth differentiation factor 15 is induced by hepatitis C virus infection and regulates hepatocellular carcinoma-related genes.
Cheng M, Gong Q, Liu X, Si Y, Wang M, Wang T, Yang W, Yang Y
PLoS ONE 2011 6:e19967
Species: Human
Sample Type: Serum
Sensitive plasma protein analysis by microparticle-based proximity ligation assays.
Alderborn A, Darmanis S, Ericsson O, Gu J, Gustafsdottir S, Hammond M, Kamali-Moghaddam M, Landegren U, Nong RY, Siegbahn A, Vanelid J
Mol. Cell Proteomics 2010 9:327-35
Species: Human
Sample Type: Plasma
Plasma hepcidin levels are elevated but responsive to erythropoietin therapy in renal disease.
Ashby DR, Bloom SR, Busbridge M, Cairns TD, Chapman RS, Choi P, Duncan ND, Gale DP, Maxwell PH, Murphy KG, Tam FW, Taube DH
Kidney Int. 2009 75:976-81
Species: Human
Sample Type: Plasma
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