Human IGFBP-2 Quantikine ELISA Kit | hIGFBP-2 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111101
R&D Systems製のHuman IGFBP-2を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月26日 00時00分現在]
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[在庫・価格 ：2025年04月26日 00時00分現在]
Human IGFBP-2 Quantikine ELISA Kit
文献数： 2
- 商品コード：DGB200
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 感度：0.09 ng/mL，測定範囲：0.313-20 ng/mL，種交差性：Human，測定試料：Serum, Plasma, Cell Culture Supernatant, Urine，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC106，測定時間：3.5 hours，測定因子：IGFBP-2 別名：BP2 Genbank No： 3485 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 | Quantikine Human IGFBP-2 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 3.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Urine (10 µL)
- Sensitivity
- 0.09 ng/mL
- Assay Range
- 0.313 - 20 ng/mL (Serum, Heparin Plasma, Cell Culture Supernates, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human IGFBP-2
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human IGFBP-2 Immunoassay is a 3.5 hour solid-phase ELISA designed to measure human IGFBP-2 in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant human IGFBP-2 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human IGFBP-2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human IGFBP-2.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 1.96 | 6.24 | 12.7 | 2.24 | 6.93 | 13.6 |
| Standard Deviation | 0.07 | 0.31 | 0.63 | 0.17 | 0.31 | 0.62 |
| CV% | 3.6 | 5 | 5 | 7.6 | 4.5 | 4.6 |
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Recovery
The recovery of IGFBP-2 spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=8) | 100 | 88-113 |
| Urine (n=4) | 109 | 94-117 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of IGFBP-2 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- IGFBP-2
- Aliases
- undefined
- Entrez Gene IDs
- 3485 (Human); 16008 (Mouse)
- Background
- IGF Binding Proteins (IGFBPs)
- The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBP, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.ALS (Acid Labile Subunit) is a liver-derived protein that exists in a ternary complex with Insulin-like Growth Factor (IGF)-binding Protein-3 (IGFBP-3) or IGFBP-5, and either IGF-I or IGF-II. ALS increases the half-life of IGF/IGFBP complexes in circulation.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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