Human FLRG Quantikine ELISA Kit | hFLRG Qkit
掲載日情報：2016/08/18 現在Webページ番号：111093
R&D Systems製のHuman FLRGを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年04月30日 08時35分現在]
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[在庫・価格 ：2024年04月30日 08時35分現在]
Human FLRG Quantikine ELISA Kit
文献数： 4
- 商品コード：DFLRG0
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
感度：8.55 pg/mL，測定範囲：62.50 - 4000 pg/mL，種交差性：Human，測定試料：Cell Culture Supernates (100 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Urine (50 uL), Human Milk (50 uL)，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC91，測定時間：4.5 hours，測定因子：Follistatin-related Gene Protein/FLRG 別名：FLRGFSRP Genbank No： 10272 |
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| 保存条件 | 4℃ | 法規備考 | |
| 掲載カタログ |
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| 製品記事 |
Quantikine Human FLRG ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Urine (50 µL), Human Milk (50 µL)
- Sensitivity
- 8.55 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL
- Specificity
- Natural and recombinant human FLRG
- Interference
- Interference observed with 1 or more available related molecules.
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Product Summary
The Quantikine Human FLRG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FLRG in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human FLRG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FLRG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FLRG.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Urine
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 468 | 1,113 | 2,252 | 494 | 1,174 | 2,331 |
| Standard Deviation | 10.4 | 23 | 55.2 | 32.9 | 69 | 143 |
| CV% | 2.2 | 2.1 | 2.5 | 6.7 | 5.9 | 6.1 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 448 | 1,094 | 2,306 | 486 | 1,148 | 2,282 |
| Standard Deviation | 15.4 | 18.5 | 49.5 | 29.5 | 53 | 122 |
| CV% | 3.4 | 1.7 | 2.1 | 6.1 | 4.6 | 5.3 |
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Recovery
The recovery of FLRG spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 107 | 98-111 |
| Serum (n=4) | 100 | 91-112 |
| EDTA Plasma (n=4) | 96 | 85-105 |
| Heparin Plasma (n=4) | 102 | 89-114 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of FLRG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- Follistatin-related Gene Protein/FLRG
- Aliases
- Follistatin-like 3; FSTL3
- Entrez Gene IDs
- 10272 (Human); 83554 (Mouse)
- Background
- Follistatin-related Gene Protein
- Follistatin-related gene protein (FLRG) has been shown to bind and inhibit the TGF-beta family ligands including activin, BMP-2, -6, -7, and GDF-8. FLRG is expressed most highly in heart, lung, kidney, and placenta, and is upregulated by TGF-beta and Activin via Smad proteins.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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