Human MFG-E8 Quantikine ELISA Kit | hMFG-E8 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111089
R&D Systems製のHuman MFG-E8を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月06日 20時55分現在]
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Human MFG-E8 Quantikine ELISA Kit |
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[在庫・価格 ：2024年05月06日 20時55分現在]
Human MFG-E8 Quantikine ELISA Kit
文献数： 8
- 商品コード：DFGE80
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
種交差性：Human，感度：13.4 pg/mL，測定範囲：62.50 - 4000 pg/mL，測定試料：Cell Culture Supernates (50 uL), Serum (20 uL), EDTA Plasma (20 uL), Heparin Plasma (20 uL), Saliva (50 uL), Urine (50 uL), Human Milk (10 uL)，測定波長：450 nm，検出方法：colorimetric，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，別売品コントロール：QC120，測定時間：4.5 hours，測定因子：MFG-E8 別名：BA46 Genbank No： 4240 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Quantikine Human MFG-E8 ELISA Kit Human Phospho-TOR (S2448) Monoclonal Antibody / APC- and PE-conjugated Mouse Axl Monoclonal Antibodies |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Saliva (50 µL), Urine (50 µL), Human Milk (10 µL)
- Sensitivity
- 13.4 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, EDTA Plasma, Urine)
- Specificity
- Natural and recombinant human MFG-E8.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure MFG-E8 in cell culture supernates, serum, plasma, saliva, urine, and human milk. It contains NS0-expressed recombinant human MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human MFG-E8. Results obtained using natural human MFG-E8 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human MFG-E8.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Serum, EDTA Plasma, Heparin Plasma, Saliva
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 645 | 1,143 | 2,168 | 749 | 1,326 | 2,339 |
| Standard Deviation | 39.5 | 46.6 | 81.1 | 57.4 | 78 | 117 |
| CV% | 6.1 | 4.1 | 3.7 | 7.7 | 5.9 | 5 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 680 | 1,181 | 2,103 | 692 | 1,262 | 2,206 |
| Standard Deviation | 30.5 | 38.9 | 84.7 | 57.6 | 88 | 125 |
| CV% | 4.5 | 3.3 | 4 | 8.3 | 7 | 5.7 |
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Recovery
The recovery of human MFG-E8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay.
| Sample Type | Average % Recovery | Range % |
| Serum (n=4) | 101 | 92-110 |
| EDTA Plasma (n=4) | 96 | 89-102 |
| Heparin Plasma (n=4) | 96 | 89-102 |
| Cell Culture Media (n=4) | 105 | 100-110 |
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Linearity
To assess the linearity of the assay, samples containing high concentrations of MFG-E8 were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- MFG-E8
- Aliases
- BA46; Breast epithelial antigen BA46; Lactahedrin; Medin; SED1
- Entrez Gene IDs
- 4240 (Human); 17304 (Mouse)
- Background
- MFG-E8
- MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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