Human FABP4 Quantikine ELISA Kit | hFABP4 Qkit
掲載日情報：2016/08/18 現在Webページ番号：111086
R&D Systems製のHuman FABP4を測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月27日 00時00分現在]
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Human FABP4 Quantikine ELISA Kit |
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[在庫・価格 ：2025年04月27日 00時00分現在]
Human FABP4 Quantikine ELISA Kit
文献数： 9
- 商品コード：DFBP40
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 | 別売品コントロール：QC186，感度：14.2 pg/mL，測定範囲：62.5-4,000 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant, Tissue Lysate，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，測定時間：4.5 hours，測定因子：FABP4, A-FABP 別名：Adipocyte lipid-binding protein Genbank No： 2167 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 | Quantikine Human FABP4 ELISA Kit ELISA Kit製品に関するFAQ（R&D Systems） |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL)
- Sensitivity
- 14.2 pg/mL
- Assay Range
- 62.5 - 4,000 pg/mL (Serum, Heparin Plasma, Cell Culture Supernates, Cell Lysates, EDTA Plasma)
- Specificity
- Natural and recombinant human FABP4.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human FABP4 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FABP4 in cell culture supernates, cell lysates, serum, and plasma. It contains E. coli-expressed recombinant human FABP4 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FABP4 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FABP4.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 349 | 1,155 | 2,595 | 407 | 1,236 | 2,619 |
| Standard Deviation | 20.2 | 39.7 | 117 | 51.6 | 156 | 291 |
| CV% | 5.8 | 3.4 | 4.5 | 12.7 | 12.6 | 11.1 |
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Recovery
The recovery of human FABP4 spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 108 | 100-118 |
| Serum (n=4) | 99 | 82-113 |
| EDTA Plasma (n=4) | 98 | 88-113 |
| Heparin Plasma (n=4) | 95 | 83-103 |
| Cell Lysis Buffer 1 (n=2) | 108 | 92-117 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human FABP4 were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
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Additional Infomation
- Molecule Information
- FABP4/A-FABP
- Aliases
- A-FABP; aP2
- Entrez Gene IDs
- 2167 (Human); 11770 (Mouse)
- Background
- FABP4
- Fatty acid binding proteins are small cytoplasmic lipid binding proteins that are expressed in a tissue specific manner. FABPs bind free fatty acids, cholesterol, and retinoids, and are involved in intracellular lipid transport. Circulating FABP levels are used as indicators of tissue damage. Some FABP polymorphisms have been associated with disorders of lipid metabolism and the development of atherosclerosis.FABP4, also known as adipocyte P2 and A-FABP (adipocyte FABP), is a FABP family member that is expressed in adipocytes and monocyte-derived foam cells. It is a lipid transport protein that binds long chain fatty acid and retinoic acid. Human and mouse FABP4 share 91% amino acid sequence homology.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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