Human Fibronectin Quantikine ELISA Kit | hFibronectin Qkit
掲載日情報：2016/08/18 現在Webページ番号：111085
R&D Systems製のHuman Fibronectinを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2024年05月06日 20時55分現在]
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[在庫・価格 ：2024年05月06日 20時55分現在]
Human Fibronectin Quantikine ELISA Kit
文献数： 1
- 商品コード：DFBN10
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：無(未発注)
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：
| 説明文 |
ヒト試料中に存在するフィブロネクチン（Fibronectin）を，サンドイッチ法により定量するELISAキット。別売品コントロール：QC200，感度：0.579 ng/mL，測定範囲：3.13 - 200 ng/mL，測定試料：Cell Culture Supernates (50 uL), Cell Lysates (50 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL), Saliva (17 uL), Urine (17 uL), Human Milk (10 uL)，種交差性：Human，検出方法：colorimetric，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96，測定時間：4.5 hours，測定因子：Fibronectin 別名：CIG Genbank No： 2335 |
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| 保存条件 | 4℃ | 法規備考 | |
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| 製品記事 |
Human Fibronectin Quantikine ELISA Kit |
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| 関連記事 |
R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL), Saliva (17 µL), Urine (17 µL), Human Milk (10 µL)
- Sensitivity
- 0.579 ng/mL
- Assay Range
- 3.13 - 200 ng/mL (Serum, Heparin Plasma, Saliva, Cell Culture Supernates, Human Milk, Cell Lysates, Citrate Plasma, EDTA Plasma, Urine)
- Specificity
- natural and recombinant human Fibronectin and Fibronectin fragments aa 1-1908, aa 607-1265, aa 627-702. Fibronectin fragment aa 631-705 cross-reacts approximately 0.415% Fibronectin fragments aa 1266-1908, aa 1722-1811, and aa 1913-2477 are not detected in this kit.
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human Fibronectin Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human Fibronectin in serum-free cell culture supernates, cell lysates, tissue lysates, serum, plasma, saliva, urine, and human milk.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians using two lots of components.
Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Saliva, Urine, Human Milk
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean | 24 | 58.1 | 123 | 23.3 | 56.5 | 115 |
| Standard Deviation | 1.4 | 2.78 | 6.35 | 1.31 | 2.98 | 6.38 |
| CV% | 5.8 | 4.8 | 5.2 | 5.6 | 5.3 | 5.5 |
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Recovery
The recovery of human Fibronectin spiked to levels throughout the range of the assay was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 97 | 89-115 |
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Linearity
To assess the linearity of the assay, samples containing and/or spiked with high concentrations of human Fibronectin were diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.
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Additional Infomation
- Molecule Information
- Fibronectin
- Aliases
- FN1
- Entrez Gene IDs
- 2335 (Human); 280794 (Bovine)
- Background
- Fibronectin
- Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions, and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, fibronectin is highly adhesive when immobilized on a surface.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
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