Human FGF basic Quantikine ELISA Kit | "hFGF basic QKit, 2nd Gen"
掲載日情報：2016/08/18 現在Webページ番号：111084
R&D Systems製のHuman FGF basicを測定するELISAキットです。
※本製品は研究用です。研究用以外には使用できません。
[在庫・価格 ：2025年04月25日 18時55分現在]
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FGF basic/FGF2, Human, ELISA Kit, Quantikine (96well) |
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[在庫・価格 ：2025年04月25日 18時55分現在]
FGF basic/FGF2, Human, ELISA Kit, Quantikine (96well)
文献数： 159
- 商品コード：DFB50
- メーカー：RSD
- 包装：1kit
- 価格：¥116,000
- 在庫：3個以上
- 納期：10日程度 ※※ 表示されている納期は弊社に在庫がなく、取り寄せた場合の目安納期となります。
- 法規制等：医薬用外毒物
| 説明文 | 測定範囲：10-640 pg/mL，感度：3 pg/mL，測定試料：Serum, Plasma, Cell Culture Supernatant，種交差性：Human，検出方法：呈色，測定波長：450 nm，アッセイ法：Solid Phase Sandwich ELISA，アッセイ数：96 well，別売品コントロール：#QC20，測定時間：4.5 hours，測定因子：FGF basic, FGF2, bFGF 別名：Basic fibroblast growth factor，bFGF Genbank No： 2247 |
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| 別包装品 | 別包装品あり | ||||||
| 法規制等 | 医薬用外毒物 | ||||||
| 保存条件 | 4℃ | 法規備考 | |||||
| 掲載カタログ |
ニュース2025年3月15日号 p.15
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| 製品記事 | R＆D Systems社のFGF Basic関連製品 ELISA Kit製品に関するFAQ（R&D Systems） 日本語マニュアル配布中！ |
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| 関連記事 | R&D Systems Quantikine ELISA Kit／DuoSet Kit特集 Quantikine ELISA Kit |
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Product Details
- Assay Type
- Solid Phase Sandwich ELISA
- Format
- 96-well strip plate
- Assay Length
- 4.5 hours
- Sample Type & Volume Required Per Well
- Cell Culture Supernates (100 µL), Serum (100 µL), EDTA Plasma (100 µL)
- Sensitivity
- 3 pg/mL
- Assay Range
- 10 - 640 pg/mL (Serum, Cell Culture Supernates, EDTA Plasma)
- Specificity
- Natural and recombinant human FGF basic
- Interference
- No significant interference observed with available related molecules.
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Product Summary
The Quantikine Human FGF basic Immunoassay kit is a 4.5 hour solid phase ELISA designed to measure human FGF basic in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant human FGF basic and antibodies raised against the recombinant factor. It has been shown to quantitate recombinant human FGF basic accurately. Results obtained using natural human FGF basic showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human FGF basic.
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Precision
Intra-Assay Precision (Precision within an assay)
Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays)
Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, EDTA Plasma
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 40 | 40 | 40 |
| Mean | 28.8 | 104 | 234 | 32.8 | 108 | 229 |
| Standard Deviation | 2.8 | 3.2 | 7.1 | 3 | 8 | 17.3 |
| CV% | 9.7 | 3.1 | 3 | 9.1 | 7.4 | 7.6 |
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Recovery
The recovery of FGF basic spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
| Cell Culture Media (n=4) | 108 | 94-119 |
| Serum (n=5) | 106 | 86-124 |
| EDTA Plasma (n=5) | 103 | 90-124 |
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Linearity
To assess the linearity of the assay, the following samples spiked with high concentrations of FGF basic were diluted with Calibrator Diluent and then assayed.
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Additional Infomation
- Molecule Information
- FGF basic
- Aliases
- FGF2; FGF-2; FGF2AS; GFG1; HBGH-2; NUDT6; Prostatropin
- Entrez Gene IDs
- 2247 (Human); 14173 (Mouse); 281161 (Bovine)
- Background
- FGF basic
- View FGF Superfamily SchematicThe Fibroblast Growth Factors (FGFs) constitute a large family of proteins involved in many aspects of development including cell proliferation, growth, and differentiation. They act on several cell types to regulate diverse physiologic functions including angiogenesis, cell growth, pattern formation, embryonic development, metabolic regulation, cell migration, neurotrophic effects, and tissue repair. FGF family activities are mediated by receptor tyrosine kinases and are facilitated by heparan sulfate. Family members have been implicated in several disorders of bone growth, as well as in tumor formation and progression.
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Citations
Citations of cell biology reagents in peer reviewed literature can be used as a direct measure of product quality. They can also provide crucial insightinto their use under specialized or unique experimental conditions. Because of the importance published citations have to researchers, R&D Systems personnelmanually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but alsoprovides information about sample types, species, and experimental conditions.
Functional and differential proteomic analyses to identify platelet derived factors affecting ex vivo expansion of mesenchymal stromal cells.
Bieback K, Dietz L, Kinzebach S, Kluter H, Thierse H
BMC Cell Biol 2013 14:48
Species: Human
Sample Type: Cell Culture Supernates
The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro.
Jin P, Li Q, Li X, Tao C, Zhang A
Biochem Biophys Res Commun 2013 441:675-80
Species: Human
Sample Type: Cell Culture Supernates
Biomarkers for antitumor activity of bevacizumab in gastric cancer models.
Fujimoto-Ouchi K, Kurasawa M, Mori K, Yamashita-Kashima Y, Yanagisawa M, Yasuno H, Yorozu K
BMC Cancer 2012 12:37
Species: Human
Sample Type: Tissue Homogenates
Differential expression of cancer associated proteins in breast milk based on age at first full term pregnancy.
Arcaro KF, Browne EP, Kliethermes B, Qin W, Ruhlen RL, Sauter ER, Zhang K
BMC Cancer 2012 12:100
Species: Human
Sample Type: Milk
Cytomegalovirus impairs cytotrophoblast-induced lymphangiogenesis and vascular remodeling in an in vivo human placentation model.
Fang-Hoover J, Inoue N, Nozawa N, Pereira L, Petitt M, Rivera J, Shiboski S, Tabata T
Am J Pathol 2012 181:1540-59
Expansion of human cardiac stem cells in physiological oxygen improves cell production efficiency and potency for myocardial repair.
Cheng K, Li TS, Malliaras K, Marban E, Marban L, Matsushita N, Sun B, Zhang Y
Cardiovasc. Res. 2011 89:157-65
Species: Human
Sample Type: Cell Culture Supernates
Fibroblast growth factor receptors are components of autocrine signaling networks in head and neck squamous cell carcinoma cells.
Bichon B, Frederick BA, Heasley LE, Hinz TK, Kono SA, Marek L, Marshall ME, Raben D, Singleton KR, Ware KE
Clin. Cancer Res. 2011 17:5016-25
Species: Human
Sample Type: Cell Lysates
In vitro vascular tube formation testing as a tool for treatment individualisation in patients with cervical cancer.
Barinoff J, Heidecke H, Korlach S, Kummel S, Landt S, Reuter C, Schwidde I, Sehouli J, Thill M
Anticancer Res. 2011 31:2609-15
Species: Human
Sample Type: Serum
Alternatives to the use of fetal bovine serum: human platelet lysates as a serum substitute in cell culture media.
Amann EM, Feifel E, Gstraunthaler G, Pfaller W, Rauch C, Schennach H, Spotl HP
ALTEX 2011 28:305-16
Species: Human
Sample Type: Cell Lysates
Platelet-derived growth factor (PDGF)-PDGF receptor interaction activates bone marrow-derived mesenchymal stromal cells derived from chronic lymphocytic leukemia: implications for an angiogenic switch.
Ding W, Knox TR, Tschumper RC
Blood 2010 116:2984-93
Species: Human
Sample Type: Platelet-Poor Plasma
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